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Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
BACKGROUND: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in huma...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429817/ https://www.ncbi.nlm.nih.gov/pubmed/25973170 http://dx.doi.org/10.1186/2045-3701-5-3 |
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author | Wang, Zhe Li, Hongqiu Zhang, Dianbao Liu, Xiaoyu Zhao, Feng Pang, Xining Wang, Qiushi |
author_facet | Wang, Zhe Li, Hongqiu Zhang, Dianbao Liu, Xiaoyu Zhao, Feng Pang, Xining Wang, Qiushi |
author_sort | Wang, Zhe |
collection | PubMed |
description | BACKGROUND: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation. RESULTS: AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK. CONCLUSIONS: These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway. |
format | Online Article Text |
id | pubmed-4429817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44298172015-05-14 Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro Wang, Zhe Li, Hongqiu Zhang, Dianbao Liu, Xiaoyu Zhao, Feng Pang, Xining Wang, Qiushi Cell Biosci Research BACKGROUND: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation. RESULTS: AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK. CONCLUSIONS: These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway. BioMed Central 2015-01-27 /pmc/articles/PMC4429817/ /pubmed/25973170 http://dx.doi.org/10.1186/2045-3701-5-3 Text en © Wang et al.; licensee BioMed Central. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Wang, Zhe Li, Hongqiu Zhang, Dianbao Liu, Xiaoyu Zhao, Feng Pang, Xining Wang, Qiushi Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
title | Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
title_full | Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
title_fullStr | Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
title_full_unstemmed | Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
title_short | Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
title_sort | effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429817/ https://www.ncbi.nlm.nih.gov/pubmed/25973170 http://dx.doi.org/10.1186/2045-3701-5-3 |
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