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Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro

BACKGROUND: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in huma...

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Autores principales: Wang, Zhe, Li, Hongqiu, Zhang, Dianbao, Liu, Xiaoyu, Zhao, Feng, Pang, Xining, Wang, Qiushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429817/
https://www.ncbi.nlm.nih.gov/pubmed/25973170
http://dx.doi.org/10.1186/2045-3701-5-3
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author Wang, Zhe
Li, Hongqiu
Zhang, Dianbao
Liu, Xiaoyu
Zhao, Feng
Pang, Xining
Wang, Qiushi
author_facet Wang, Zhe
Li, Hongqiu
Zhang, Dianbao
Liu, Xiaoyu
Zhao, Feng
Pang, Xining
Wang, Qiushi
author_sort Wang, Zhe
collection PubMed
description BACKGROUND: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation. RESULTS: AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK. CONCLUSIONS: These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway.
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spelling pubmed-44298172015-05-14 Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro Wang, Zhe Li, Hongqiu Zhang, Dianbao Liu, Xiaoyu Zhao, Feng Pang, Xining Wang, Qiushi Cell Biosci Research BACKGROUND: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation. RESULTS: AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK. CONCLUSIONS: These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway. BioMed Central 2015-01-27 /pmc/articles/PMC4429817/ /pubmed/25973170 http://dx.doi.org/10.1186/2045-3701-5-3 Text en © Wang et al.; licensee BioMed Central. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Zhe
Li, Hongqiu
Zhang, Dianbao
Liu, Xiaoyu
Zhao, Feng
Pang, Xining
Wang, Qiushi
Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
title Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
title_full Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
title_fullStr Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
title_full_unstemmed Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
title_short Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
title_sort effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429817/
https://www.ncbi.nlm.nih.gov/pubmed/25973170
http://dx.doi.org/10.1186/2045-3701-5-3
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