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Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study
BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regula...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429932/ https://www.ncbi.nlm.nih.gov/pubmed/25971815 http://dx.doi.org/10.1186/1476-511X-14-4 |
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author | Schmidt, Simone Willers, Janina Riecker, Sabine Möller, Katharina Schuchardt, Jan Philipp Hahn, Andreas |
author_facet | Schmidt, Simone Willers, Janina Riecker, Sabine Möller, Katharina Schuchardt, Jan Philipp Hahn, Andreas |
author_sort | Schmidt, Simone |
collection | PubMed |
description | BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level. METHODS: A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging. RESULTS: N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation. CONCLUSIONS: Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1476-511X-14-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4429932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44299322015-05-14 Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study Schmidt, Simone Willers, Janina Riecker, Sabine Möller, Katharina Schuchardt, Jan Philipp Hahn, Andreas Lipids Health Dis Research BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level. METHODS: A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging. RESULTS: N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation. CONCLUSIONS: Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1476-511X-14-4) contains supplementary material, which is available to authorized users. BioMed Central 2015-02-14 /pmc/articles/PMC4429932/ /pubmed/25971815 http://dx.doi.org/10.1186/1476-511X-14-4 Text en © Schmidt et al.; licensee BioMed Central. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Schmidt, Simone Willers, Janina Riecker, Sabine Möller, Katharina Schuchardt, Jan Philipp Hahn, Andreas Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
title | Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
title_full | Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
title_fullStr | Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
title_full_unstemmed | Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
title_short | Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
title_sort | effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429932/ https://www.ncbi.nlm.nih.gov/pubmed/25971815 http://dx.doi.org/10.1186/1476-511X-14-4 |
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