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The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice
A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant h...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430236/ https://www.ncbi.nlm.nih.gov/pubmed/25970642 http://dx.doi.org/10.1371/journal.pone.0126186 |
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author | Ye, Ying Yuan, Jing Chang, Xiaojian Yang, Meng Zhang, Lejing Lu, Kai Lian, Xingming |
author_facet | Ye, Ying Yuan, Jing Chang, Xiaojian Yang, Meng Zhang, Lejing Lu, Kai Lian, Xingming |
author_sort | Ye, Ying |
collection | PubMed |
description | A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice. Expression of the β-glucuronidase reporter gene driven by the OsPT4 promoter showed that OsPT4 is expressed in roots, leaves, ligules, stamens, and caryopses under sufficient Pi conditions, consistent with the expression profile showing that OsPT4 has high expression in roots and flag leaves. The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation. OsPT4 encoded a plasma membrane—localized protein and was able to complement the function of the Pi transporter gene PHO84 in yeast. We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation. This study will enrich our understanding about the physiological function of rice Pht1 family genes. |
format | Online Article Text |
id | pubmed-4430236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44302362015-05-21 The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice Ye, Ying Yuan, Jing Chang, Xiaojian Yang, Meng Zhang, Lejing Lu, Kai Lian, Xingming PLoS One Research Article A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice. Expression of the β-glucuronidase reporter gene driven by the OsPT4 promoter showed that OsPT4 is expressed in roots, leaves, ligules, stamens, and caryopses under sufficient Pi conditions, consistent with the expression profile showing that OsPT4 has high expression in roots and flag leaves. The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation. OsPT4 encoded a plasma membrane—localized protein and was able to complement the function of the Pi transporter gene PHO84 in yeast. We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation. This study will enrich our understanding about the physiological function of rice Pht1 family genes. Public Library of Science 2015-05-13 /pmc/articles/PMC4430236/ /pubmed/25970642 http://dx.doi.org/10.1371/journal.pone.0126186 Text en © 2015 Ye et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ye, Ying Yuan, Jing Chang, Xiaojian Yang, Meng Zhang, Lejing Lu, Kai Lian, Xingming The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice |
title | The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice |
title_full | The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice |
title_fullStr | The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice |
title_full_unstemmed | The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice |
title_short | The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice |
title_sort | phosphate transporter gene ospht1;4 is involved in phosphate homeostasis in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430236/ https://www.ncbi.nlm.nih.gov/pubmed/25970642 http://dx.doi.org/10.1371/journal.pone.0126186 |
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