Cargando…

Do Housekeeping Genes Exist?

The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yijuan, Li, Ding, Sun, Bingyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430495/
https://www.ncbi.nlm.nih.gov/pubmed/25970694
http://dx.doi.org/10.1371/journal.pone.0123691
_version_ 1782371182748631040
author Zhang, Yijuan
Li, Ding
Sun, Bingyun
author_facet Zhang, Yijuan
Li, Ding
Sun, Bingyun
author_sort Zhang, Yijuan
collection PubMed
description The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization standards in quantitative gene-expression analysis. Within the past 20 years, more than a dozen HK-gene studies have been conducted, yet none of them sampled human tissues completely. We believe an integration of these results will help remove false positive genes owing to the inadequate sampling. Surprisingly, we only find one common gene across 15 examined HK-gene datasets comprising 187 different tissue and cell types. Our subsequent analyses suggest that it might not be appropriate to rigidly define HK genes as expressed in all tissue types that have diverse developmental, physiological, and pathological states. It might be beneficial to use more robustly identified HK functions for filtering criteria, in which the representing genes can be a subset of genome. These genes are not necessarily the same, and perhaps need not to be the same, everywhere in our body.
format Online
Article
Text
id pubmed-4430495
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44304952015-05-21 Do Housekeeping Genes Exist? Zhang, Yijuan Li, Ding Sun, Bingyun PLoS One Research Article The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization standards in quantitative gene-expression analysis. Within the past 20 years, more than a dozen HK-gene studies have been conducted, yet none of them sampled human tissues completely. We believe an integration of these results will help remove false positive genes owing to the inadequate sampling. Surprisingly, we only find one common gene across 15 examined HK-gene datasets comprising 187 different tissue and cell types. Our subsequent analyses suggest that it might not be appropriate to rigidly define HK genes as expressed in all tissue types that have diverse developmental, physiological, and pathological states. It might be beneficial to use more robustly identified HK functions for filtering criteria, in which the representing genes can be a subset of genome. These genes are not necessarily the same, and perhaps need not to be the same, everywhere in our body. Public Library of Science 2015-05-13 /pmc/articles/PMC4430495/ /pubmed/25970694 http://dx.doi.org/10.1371/journal.pone.0123691 Text en © 2015 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Yijuan
Li, Ding
Sun, Bingyun
Do Housekeeping Genes Exist?
title Do Housekeeping Genes Exist?
title_full Do Housekeeping Genes Exist?
title_fullStr Do Housekeeping Genes Exist?
title_full_unstemmed Do Housekeeping Genes Exist?
title_short Do Housekeeping Genes Exist?
title_sort do housekeeping genes exist?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430495/
https://www.ncbi.nlm.nih.gov/pubmed/25970694
http://dx.doi.org/10.1371/journal.pone.0123691
work_keys_str_mv AT zhangyijuan dohousekeepinggenesexist
AT liding dohousekeepinggenesexist
AT sunbingyun dohousekeepinggenesexist