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Expression of the vault RNA protects cells from undergoing apoptosis
Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein–Barr virus (EBV)-infected human B cells. Very little is known...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430821/ https://www.ncbi.nlm.nih.gov/pubmed/25952297 http://dx.doi.org/10.1038/ncomms8030 |
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author | Amort, Melanie Nachbauer, Birgit Tuzlak, Selma Kieser, Arnd Schepers, Aloys Villunger, Andreas Polacek, Norbert |
author_facet | Amort, Melanie Nachbauer, Birgit Tuzlak, Selma Kieser, Arnd Schepers, Aloys Villunger, Andreas Polacek, Norbert |
author_sort | Amort, Melanie |
collection | PubMed |
description | Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein–Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation. Ectopic expression of vtRNA1-1, but not of the other vtRNA paralogues, results in an improved viral establishment and reduced apoptosis, a function located in the central domain of vtRNA1-1. Knockdown of the major vault protein has no effect on these phenotypes revealing that vtRNA1-1 and not the vault complex contributes to general cell death resistance. This study describes a NF-κB-mediated role of the non-coding vtRNA1-1 in inhibiting both the extrinsic and intrinsic apoptotic pathways. |
format | Online Article Text |
id | pubmed-4430821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44308212015-05-23 Expression of the vault RNA protects cells from undergoing apoptosis Amort, Melanie Nachbauer, Birgit Tuzlak, Selma Kieser, Arnd Schepers, Aloys Villunger, Andreas Polacek, Norbert Nat Commun Article Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein–Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation. Ectopic expression of vtRNA1-1, but not of the other vtRNA paralogues, results in an improved viral establishment and reduced apoptosis, a function located in the central domain of vtRNA1-1. Knockdown of the major vault protein has no effect on these phenotypes revealing that vtRNA1-1 and not the vault complex contributes to general cell death resistance. This study describes a NF-κB-mediated role of the non-coding vtRNA1-1 in inhibiting both the extrinsic and intrinsic apoptotic pathways. Nature Pub. Group 2015-05-08 /pmc/articles/PMC4430821/ /pubmed/25952297 http://dx.doi.org/10.1038/ncomms8030 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Amort, Melanie Nachbauer, Birgit Tuzlak, Selma Kieser, Arnd Schepers, Aloys Villunger, Andreas Polacek, Norbert Expression of the vault RNA protects cells from undergoing apoptosis |
title | Expression of the vault RNA protects cells from undergoing apoptosis |
title_full | Expression of the vault RNA protects cells from undergoing apoptosis |
title_fullStr | Expression of the vault RNA protects cells from undergoing apoptosis |
title_full_unstemmed | Expression of the vault RNA protects cells from undergoing apoptosis |
title_short | Expression of the vault RNA protects cells from undergoing apoptosis |
title_sort | expression of the vault rna protects cells from undergoing apoptosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430821/ https://www.ncbi.nlm.nih.gov/pubmed/25952297 http://dx.doi.org/10.1038/ncomms8030 |
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