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Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots

Studying gene expression profile in a single cancer cell is important because multiple genes are associated with cancer development. Quantum dots (QDs) have been utilized as biological probes for imaging and detection. QDs display specific optical and electrical properties that depend on their size...

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Detalles Bibliográficos
Autores principales: Lee, Jonghwan, Kang, Hyo Jin, Jang, Hyeok, Lee, Youn Jung, Lee, Yong Seung, Ali, Bahy A., Al-Khedhairy, Abdulaziz A., Kim, Soonhag
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431267/
https://www.ncbi.nlm.nih.gov/pubmed/25871725
http://dx.doi.org/10.3390/s150408595
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author Lee, Jonghwan
Kang, Hyo Jin
Jang, Hyeok
Lee, Youn Jung
Lee, Yong Seung
Ali, Bahy A.
Al-Khedhairy, Abdulaziz A.
Kim, Soonhag
author_facet Lee, Jonghwan
Kang, Hyo Jin
Jang, Hyeok
Lee, Youn Jung
Lee, Yong Seung
Ali, Bahy A.
Al-Khedhairy, Abdulaziz A.
Kim, Soonhag
author_sort Lee, Jonghwan
collection PubMed
description Studying gene expression profile in a single cancer cell is important because multiple genes are associated with cancer development. Quantum dots (QDs) have been utilized as biological probes for imaging and detection. QDs display specific optical and electrical properties that depend on their size that can be applied for imaging and sensing applications. In this study, simultaneous imaging of the cancer biomarkers, tenascin-C and nucleolin, was performed using two types of aptamer-conjugated QDs. The simultaneous imaging of these two different cancer markers in three cancer cell lines was reliable and cell line-specific. Current requirements for cancer imaging technologies include the need for simple preparation methods and the ability to detect multiple cancer biomarkers and evaluate their intracellular localizations. The method employed in this study is a feasible solution to these requirements.
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spelling pubmed-44312672015-05-19 Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots Lee, Jonghwan Kang, Hyo Jin Jang, Hyeok Lee, Youn Jung Lee, Yong Seung Ali, Bahy A. Al-Khedhairy, Abdulaziz A. Kim, Soonhag Sensors (Basel) Article Studying gene expression profile in a single cancer cell is important because multiple genes are associated with cancer development. Quantum dots (QDs) have been utilized as biological probes for imaging and detection. QDs display specific optical and electrical properties that depend on their size that can be applied for imaging and sensing applications. In this study, simultaneous imaging of the cancer biomarkers, tenascin-C and nucleolin, was performed using two types of aptamer-conjugated QDs. The simultaneous imaging of these two different cancer markers in three cancer cell lines was reliable and cell line-specific. Current requirements for cancer imaging technologies include the need for simple preparation methods and the ability to detect multiple cancer biomarkers and evaluate their intracellular localizations. The method employed in this study is a feasible solution to these requirements. MDPI 2015-04-13 /pmc/articles/PMC4431267/ /pubmed/25871725 http://dx.doi.org/10.3390/s150408595 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jonghwan
Kang, Hyo Jin
Jang, Hyeok
Lee, Youn Jung
Lee, Yong Seung
Ali, Bahy A.
Al-Khedhairy, Abdulaziz A.
Kim, Soonhag
Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots
title Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots
title_full Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots
title_fullStr Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots
title_full_unstemmed Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots
title_short Simultaneous Imaging of Two Different Cancer Biomarkers Using Aptamer-Conjugated Quantum Dots
title_sort simultaneous imaging of two different cancer biomarkers using aptamer-conjugated quantum dots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431267/
https://www.ncbi.nlm.nih.gov/pubmed/25871725
http://dx.doi.org/10.3390/s150408595
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