An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine

Because of the biocompatible properties of gelatine and the good affinity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gela...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamidi-Asl, Ezat, Dardenne, Freddy, Blust, Ronny, De Wael, Karolien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431305/
https://www.ncbi.nlm.nih.gov/pubmed/25825978
http://dx.doi.org/10.3390/s150407605
_version_ 1782371320692998144
author Hamidi-Asl, Ezat
Dardenne, Freddy
Blust, Ronny
De Wael, Karolien
author_facet Hamidi-Asl, Ezat
Dardenne, Freddy
Blust, Ronny
De Wael, Karolien
author_sort Hamidi-Asl, Ezat
collection PubMed
description Because of the biocompatible properties of gelatine and the good affinity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 × 10(−10) M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature.
format Online
Article
Text
id pubmed-4431305
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-44313052015-05-19 An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine Hamidi-Asl, Ezat Dardenne, Freddy Blust, Ronny De Wael, Karolien Sensors (Basel) Article Because of the biocompatible properties of gelatine and the good affinity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 × 10(−10) M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature. MDPI 2015-03-27 /pmc/articles/PMC4431305/ /pubmed/25825978 http://dx.doi.org/10.3390/s150407605 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hamidi-Asl, Ezat
Dardenne, Freddy
Blust, Ronny
De Wael, Karolien
An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine
title An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine
title_full An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine
title_fullStr An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine
title_full_unstemmed An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine
title_short An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine
title_sort improved electrochemical aptasensor for chloramphenicol detection based on aptamer incorporated gelatine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431305/
https://www.ncbi.nlm.nih.gov/pubmed/25825978
http://dx.doi.org/10.3390/s150407605
work_keys_str_mv AT hamidiaslezat animprovedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT dardennefreddy animprovedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT blustronny animprovedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT dewaelkarolien animprovedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT hamidiaslezat improvedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT dardennefreddy improvedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT blustronny improvedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine
AT dewaelkarolien improvedelectrochemicalaptasensorforchloramphenicoldetectionbasedonaptamerincorporatedgelatine

Ejemplares similares