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Lowering the quantification limit of the Qubit(TM) RNA HS Assay using RNA spike-in

BACKGROUND: RNA quantification is often a prerequisite for most RNA analyses such as RNA sequencing. However, the relatively low sensitivity and large sample consumption of traditional RNA quantification methods such as UV spectrophotometry and even the much more sensitive fluorescence-based RNA qua...

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Detalles Bibliográficos
Autores principales: Li, Xin, Ben-Dov, Iddo Z, Mauro, Maurizio, Williams, Zev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431604/
https://www.ncbi.nlm.nih.gov/pubmed/25943882
http://dx.doi.org/10.1186/s12867-015-0039-3