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Est10: A Novel Alkaline Esterase Isolated from Bovine Rumen Belonging to the New Family XV of Lipolytic Enzymes

A metagenomic fosmid library from bovine rumen was used to identify clones with lipolytic activity. One positive clone was isolated. The gene responsible for the observed phenotype was identified by in vitro transposon mutagenesis and sequencing and was named est10. The 367 amino acids sequence harb...

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Detalles Bibliográficos
Autores principales: Rodríguez, María Cecilia, Loaces, Inés, Amarelle, Vanesa, Senatore, Daniella, Iriarte, Andrés, Fabiano, Elena, Noya, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431682/
https://www.ncbi.nlm.nih.gov/pubmed/25973851
http://dx.doi.org/10.1371/journal.pone.0126651