Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff po...

Descripción completa

Detalles Bibliográficos
Autores principales: Bennett, A., Bar-Zeev, N., Jere, K. C., Tate, J. E., Parashar, U. D., Nakagomi, O., Heyderman, R. S., French, N., Iturriza-Gomara, M., Cunliffe, N. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432065/
https://www.ncbi.nlm.nih.gov/pubmed/25854480
http://dx.doi.org/10.1128/JCM.00875-15
Descripción
Sumario:We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.

Ejemplares similares