Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff po...

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Detalles Bibliográficos
Autores principales: Bennett, A., Bar-Zeev, N., Jere, K. C., Tate, J. E., Parashar, U. D., Nakagomi, O., Heyderman, R. S., French, N., Iturriza-Gomara, M., Cunliffe, N. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432065/
https://www.ncbi.nlm.nih.gov/pubmed/25854480
http://dx.doi.org/10.1128/JCM.00875-15
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author Bennett, A.
Bar-Zeev, N.
Jere, K. C.
Tate, J. E.
Parashar, U. D.
Nakagomi, O.
Heyderman, R. S.
French, N.
Iturriza-Gomara, M.
Cunliffe, N. A.
author_facet Bennett, A.
Bar-Zeev, N.
Jere, K. C.
Tate, J. E.
Parashar, U. D.
Nakagomi, O.
Heyderman, R. S.
French, N.
Iturriza-Gomara, M.
Cunliffe, N. A.
author_sort Bennett, A.
collection PubMed
description We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.
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spelling pubmed-44320652015-05-30 Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population Bennett, A. Bar-Zeev, N. Jere, K. C. Tate, J. E. Parashar, U. D. Nakagomi, O. Heyderman, R. S. French, N. Iturriza-Gomara, M. Cunliffe, N. A. J Clin Microbiol Virology We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa. American Society for Microbiology 2015-05-14 2015-06 /pmc/articles/PMC4432065/ /pubmed/25854480 http://dx.doi.org/10.1128/JCM.00875-15 Text en Copyright © 2015, Bennett et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Virology
Bennett, A.
Bar-Zeev, N.
Jere, K. C.
Tate, J. E.
Parashar, U. D.
Nakagomi, O.
Heyderman, R. S.
French, N.
Iturriza-Gomara, M.
Cunliffe, N. A.
Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population
title Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population
title_full Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population
title_fullStr Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population
title_full_unstemmed Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population
title_short Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population
title_sort determination of a viral load threshold to distinguish symptomatic versus asymptomatic rotavirus infection in a high-disease-burden african population
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432065/
https://www.ncbi.nlm.nih.gov/pubmed/25854480
http://dx.doi.org/10.1128/JCM.00875-15
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