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Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays
Engineered nanoparticles (eNPs) for biological and biomedical applications are produced from functionalised nanoparticles (NPs) after undergoing multiple handling steps, giving rise to an inevitable loss of NPs. Herein we present a practical method to quantify nanoparticles (NPs) number per volume i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432369/ https://www.ncbi.nlm.nih.gov/pubmed/25976173 http://dx.doi.org/10.1038/srep10091 |
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author | Unciti-Broceta, Juan D. Cano-Cortés, Victoria Altea-Manzano, Patricia Pernagallo, Salvatore Díaz-Mochón, Juan J. Sánchez-Martín, Rosario M. |
author_facet | Unciti-Broceta, Juan D. Cano-Cortés, Victoria Altea-Manzano, Patricia Pernagallo, Salvatore Díaz-Mochón, Juan J. Sánchez-Martín, Rosario M. |
author_sort | Unciti-Broceta, Juan D. |
collection | PubMed |
description | Engineered nanoparticles (eNPs) for biological and biomedical applications are produced from functionalised nanoparticles (NPs) after undergoing multiple handling steps, giving rise to an inevitable loss of NPs. Herein we present a practical method to quantify nanoparticles (NPs) number per volume in an aqueous suspension using standard spectrophotometers and minute amounts of the suspensions (up to 1 μL). This method allows, for the first time, to analyse cellular uptake by reporting NPs number added per cell, as opposed to current methods which are related to solid content (w/V) of NPs. In analogy to the parameter used in viral infective assays (multiplicity of infection), we propose to name this novel parameter as multiplicity of nanofection. |
format | Online Article Text |
id | pubmed-4432369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44323692015-05-22 Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays Unciti-Broceta, Juan D. Cano-Cortés, Victoria Altea-Manzano, Patricia Pernagallo, Salvatore Díaz-Mochón, Juan J. Sánchez-Martín, Rosario M. Sci Rep Article Engineered nanoparticles (eNPs) for biological and biomedical applications are produced from functionalised nanoparticles (NPs) after undergoing multiple handling steps, giving rise to an inevitable loss of NPs. Herein we present a practical method to quantify nanoparticles (NPs) number per volume in an aqueous suspension using standard spectrophotometers and minute amounts of the suspensions (up to 1 μL). This method allows, for the first time, to analyse cellular uptake by reporting NPs number added per cell, as opposed to current methods which are related to solid content (w/V) of NPs. In analogy to the parameter used in viral infective assays (multiplicity of infection), we propose to name this novel parameter as multiplicity of nanofection. Nature Publishing Group 2015-05-15 /pmc/articles/PMC4432369/ /pubmed/25976173 http://dx.doi.org/10.1038/srep10091 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Unciti-Broceta, Juan D. Cano-Cortés, Victoria Altea-Manzano, Patricia Pernagallo, Salvatore Díaz-Mochón, Juan J. Sánchez-Martín, Rosario M. Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays |
title | Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays |
title_full | Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays |
title_fullStr | Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays |
title_full_unstemmed | Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays |
title_short | Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays |
title_sort | number of nanoparticles per cell through a spectrophotometric method - a key parameter to assess nanoparticle-based cellular assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432369/ https://www.ncbi.nlm.nih.gov/pubmed/25976173 http://dx.doi.org/10.1038/srep10091 |
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