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Cytochrome P450 in living donor liver transplantation
Cytochrome P450 metabolizes many drugs in the liver. Three genotypes of CYP2C19 with extensive, intermediate, and poor metabolizing activity, respectively, have been identified in peripheral blood of transplant recipients and new liver grafts in living donor liver transplantation (LDLT). The express...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432787/ https://www.ncbi.nlm.nih.gov/pubmed/25975271 http://dx.doi.org/10.1186/s12929-015-0140-4 |
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author | Chiu, King-Wah Nakano, Toshiaki Chen, Kuang-Den Hsu, Li-Wen Lai, Chia-Yun Huang, Ching-Yin Cheng, Yu-Fan Goto, Shigeru Chen, Chao-Long |
author_facet | Chiu, King-Wah Nakano, Toshiaki Chen, Kuang-Den Hsu, Li-Wen Lai, Chia-Yun Huang, Ching-Yin Cheng, Yu-Fan Goto, Shigeru Chen, Chao-Long |
author_sort | Chiu, King-Wah |
collection | PubMed |
description | Cytochrome P450 metabolizes many drugs in the liver. Three genotypes of CYP2C19 with extensive, intermediate, and poor metabolizing activity, respectively, have been identified in peripheral blood of transplant recipients and new liver grafts in living donor liver transplantation (LDLT). The expression of the final genotype in liver graft biopsies depends on the donor, whereas the expression in peripheral blood mononuclear cells depends on the recipient. The metabolizing isoenzyme of the major anti-rejection agents passes through CYP3A4, CYP3A5 and MDR1, which have also been identified to have similar biological characteristics as genotype of CYP2C19 in liver tissue. Recently, pyrosequencing has been used to investigate the expressions of different genotypes in liver grafts in LDLT. This review focuses on recent findings regarding the biological expressions of the CYP2C19, CYP3A4, CYP3A5 and MRD1 genotypes in liver grafts before and after LDLT. The application of pyrosequencing may be beneficial in further research on liver transplantation. Laser capture microdissection of hepatocytes in liver grafts may be a direction for future research. |
format | Online Article Text |
id | pubmed-4432787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44327872015-05-16 Cytochrome P450 in living donor liver transplantation Chiu, King-Wah Nakano, Toshiaki Chen, Kuang-Den Hsu, Li-Wen Lai, Chia-Yun Huang, Ching-Yin Cheng, Yu-Fan Goto, Shigeru Chen, Chao-Long J Biomed Sci Review Cytochrome P450 metabolizes many drugs in the liver. Three genotypes of CYP2C19 with extensive, intermediate, and poor metabolizing activity, respectively, have been identified in peripheral blood of transplant recipients and new liver grafts in living donor liver transplantation (LDLT). The expression of the final genotype in liver graft biopsies depends on the donor, whereas the expression in peripheral blood mononuclear cells depends on the recipient. The metabolizing isoenzyme of the major anti-rejection agents passes through CYP3A4, CYP3A5 and MDR1, which have also been identified to have similar biological characteristics as genotype of CYP2C19 in liver tissue. Recently, pyrosequencing has been used to investigate the expressions of different genotypes in liver grafts in LDLT. This review focuses on recent findings regarding the biological expressions of the CYP2C19, CYP3A4, CYP3A5 and MRD1 genotypes in liver grafts before and after LDLT. The application of pyrosequencing may be beneficial in further research on liver transplantation. Laser capture microdissection of hepatocytes in liver grafts may be a direction for future research. BioMed Central 2015-05-15 /pmc/articles/PMC4432787/ /pubmed/25975271 http://dx.doi.org/10.1186/s12929-015-0140-4 Text en © Chiu et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Chiu, King-Wah Nakano, Toshiaki Chen, Kuang-Den Hsu, Li-Wen Lai, Chia-Yun Huang, Ching-Yin Cheng, Yu-Fan Goto, Shigeru Chen, Chao-Long Cytochrome P450 in living donor liver transplantation |
title | Cytochrome P450 in living donor liver transplantation |
title_full | Cytochrome P450 in living donor liver transplantation |
title_fullStr | Cytochrome P450 in living donor liver transplantation |
title_full_unstemmed | Cytochrome P450 in living donor liver transplantation |
title_short | Cytochrome P450 in living donor liver transplantation |
title_sort | cytochrome p450 in living donor liver transplantation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432787/ https://www.ncbi.nlm.nih.gov/pubmed/25975271 http://dx.doi.org/10.1186/s12929-015-0140-4 |
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