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Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples

Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface a...

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Autores principales: Tveen-Jensen, Karina, Gesellchen, Frank, Wilson, Rab, Spickett, Corinne M., Cooper, Jonathan M., Pitt, Andrew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432867/
https://www.ncbi.nlm.nih.gov/pubmed/25978651
http://dx.doi.org/10.1038/srep09736
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author Tveen-Jensen, Karina
Gesellchen, Frank
Wilson, Rab
Spickett, Corinne M.
Cooper, Jonathan M.
Pitt, Andrew R.
author_facet Tveen-Jensen, Karina
Gesellchen, Frank
Wilson, Rab
Spickett, Corinne M.
Cooper, Jonathan M.
Pitt, Andrew R.
author_sort Tveen-Jensen, Karina
collection PubMed
description Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.
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spelling pubmed-44328672015-05-22 Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples Tveen-Jensen, Karina Gesellchen, Frank Wilson, Rab Spickett, Corinne M. Cooper, Jonathan M. Pitt, Andrew R. Sci Rep Article Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples. Nature Publishing Group 2015-05-15 /pmc/articles/PMC4432867/ /pubmed/25978651 http://dx.doi.org/10.1038/srep09736 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tveen-Jensen, Karina
Gesellchen, Frank
Wilson, Rab
Spickett, Corinne M.
Cooper, Jonathan M.
Pitt, Andrew R.
Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples
title Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples
title_full Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples
title_fullStr Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples
title_full_unstemmed Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples
title_short Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples
title_sort interfacing low-energy saw nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432867/
https://www.ncbi.nlm.nih.gov/pubmed/25978651
http://dx.doi.org/10.1038/srep09736
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