Cargando…
Chromosomal instability as a prognostic marker in cervical cancer
BACKGROUND: Cervical cancer is the third most common cancer in women globally, and despite treatment, distant metastasis and nodal recurrence will still develop in approximately 30% of patients. The ability to predict which patients are likely to experience distant relapse would allow clinicians to...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433070/ https://www.ncbi.nlm.nih.gov/pubmed/25944123 http://dx.doi.org/10.1186/s12885-015-1372-0 |
_version_ | 1782371576046419968 |
---|---|
author | How, Christine Bruce, Jeff So, Jonathan Pintilie, Melania Haibe-Kains, Benjamin Hui, Angela Clarke, Blaise A Hedley, David W Hill, Richard P Milosevic, Michael Fyles, Anthony Liu, Fei-Fei |
author_facet | How, Christine Bruce, Jeff So, Jonathan Pintilie, Melania Haibe-Kains, Benjamin Hui, Angela Clarke, Blaise A Hedley, David W Hill, Richard P Milosevic, Michael Fyles, Anthony Liu, Fei-Fei |
author_sort | How, Christine |
collection | PubMed |
description | BACKGROUND: Cervical cancer is the third most common cancer in women globally, and despite treatment, distant metastasis and nodal recurrence will still develop in approximately 30% of patients. The ability to predict which patients are likely to experience distant relapse would allow clinicians to better tailor treatment. Previous studies have investigated the role of chromosomal instability (CIN) in cancer, which can promote tumour initiation and growth; a hallmark of human malignancies. In this study, we sought to examine the published CIN70 gene signature in a cohort of cervical cancer patients treated at the Princess Margaret (PM) Cancer Centre and an independent cohort of The Cancer Genome Atlas (TCGA) cervical cancer patients, to determine if this CIN signature associated with patient outcome. METHODS: Cervical cancer samples were collected from 79 patients, treated between 2000–2007 at the PM, prior to undergoing curative chemo-radiation. Total RNA was extracted from each patient sample and analyzed using the GeneChip Human Genome U133 Plus 2.0 array (Affymetrix). RESULTS: High CIN70 scores were significantly related to increased chromosomal alterations in TCGA cervical cancer patients, including a higher percentage of genome altered and a higher number of copy number alterations. In addition, this same CIN70 signature was shown to be predictive of para-aortic nodal relapse in the PM Cancer Centre cohort. CONCLUSIONS: These findings demonstrate that chromosomal instability plays an important role in cervical cancer, and is significantly associated with patient outcome. For the first time, this CIN70 gene signature provided prognostic value for patients with cervical cancer. |
format | Online Article Text |
id | pubmed-4433070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44330702015-05-16 Chromosomal instability as a prognostic marker in cervical cancer How, Christine Bruce, Jeff So, Jonathan Pintilie, Melania Haibe-Kains, Benjamin Hui, Angela Clarke, Blaise A Hedley, David W Hill, Richard P Milosevic, Michael Fyles, Anthony Liu, Fei-Fei BMC Cancer Research Article BACKGROUND: Cervical cancer is the third most common cancer in women globally, and despite treatment, distant metastasis and nodal recurrence will still develop in approximately 30% of patients. The ability to predict which patients are likely to experience distant relapse would allow clinicians to better tailor treatment. Previous studies have investigated the role of chromosomal instability (CIN) in cancer, which can promote tumour initiation and growth; a hallmark of human malignancies. In this study, we sought to examine the published CIN70 gene signature in a cohort of cervical cancer patients treated at the Princess Margaret (PM) Cancer Centre and an independent cohort of The Cancer Genome Atlas (TCGA) cervical cancer patients, to determine if this CIN signature associated with patient outcome. METHODS: Cervical cancer samples were collected from 79 patients, treated between 2000–2007 at the PM, prior to undergoing curative chemo-radiation. Total RNA was extracted from each patient sample and analyzed using the GeneChip Human Genome U133 Plus 2.0 array (Affymetrix). RESULTS: High CIN70 scores were significantly related to increased chromosomal alterations in TCGA cervical cancer patients, including a higher percentage of genome altered and a higher number of copy number alterations. In addition, this same CIN70 signature was shown to be predictive of para-aortic nodal relapse in the PM Cancer Centre cohort. CONCLUSIONS: These findings demonstrate that chromosomal instability plays an important role in cervical cancer, and is significantly associated with patient outcome. For the first time, this CIN70 gene signature provided prognostic value for patients with cervical cancer. BioMed Central 2015-05-06 /pmc/articles/PMC4433070/ /pubmed/25944123 http://dx.doi.org/10.1186/s12885-015-1372-0 Text en © How et al.; licensee BioMed Central. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article How, Christine Bruce, Jeff So, Jonathan Pintilie, Melania Haibe-Kains, Benjamin Hui, Angela Clarke, Blaise A Hedley, David W Hill, Richard P Milosevic, Michael Fyles, Anthony Liu, Fei-Fei Chromosomal instability as a prognostic marker in cervical cancer |
title | Chromosomal instability as a prognostic marker in cervical cancer |
title_full | Chromosomal instability as a prognostic marker in cervical cancer |
title_fullStr | Chromosomal instability as a prognostic marker in cervical cancer |
title_full_unstemmed | Chromosomal instability as a prognostic marker in cervical cancer |
title_short | Chromosomal instability as a prognostic marker in cervical cancer |
title_sort | chromosomal instability as a prognostic marker in cervical cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433070/ https://www.ncbi.nlm.nih.gov/pubmed/25944123 http://dx.doi.org/10.1186/s12885-015-1372-0 |
work_keys_str_mv | AT howchristine chromosomalinstabilityasaprognosticmarkerincervicalcancer AT brucejeff chromosomalinstabilityasaprognosticmarkerincervicalcancer AT sojonathan chromosomalinstabilityasaprognosticmarkerincervicalcancer AT pintiliemelania chromosomalinstabilityasaprognosticmarkerincervicalcancer AT haibekainsbenjamin chromosomalinstabilityasaprognosticmarkerincervicalcancer AT huiangela chromosomalinstabilityasaprognosticmarkerincervicalcancer AT clarkeblaisea chromosomalinstabilityasaprognosticmarkerincervicalcancer AT hedleydavidw chromosomalinstabilityasaprognosticmarkerincervicalcancer AT hillrichardp chromosomalinstabilityasaprognosticmarkerincervicalcancer AT milosevicmichael chromosomalinstabilityasaprognosticmarkerincervicalcancer AT fylesanthony chromosomalinstabilityasaprognosticmarkerincervicalcancer AT liufeifei chromosomalinstabilityasaprognosticmarkerincervicalcancer |