Cargando…

Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname

Guyana and Suriname have made important progress in reducing the burden of malaria. While both countries use microscopy as the primary tool for clinical diagnosis, malaria rapid diagnostic tests (RDTs) are useful in remote areas of the interior where laboratory support may be limited or unavailable....

Descripción completa

Detalles Bibliográficos
Autores principales: Akinyi Okoth, Sheila, Abdallah, Joseph F., Ceron, Nicolas, Adhin, Malti R., Chandrabose, Javin, Krishnalall, Karanchand, Huber, Curtis S., Goldman, Ira F., Macedo de Oliveira, Alexandre, Barnwell, John W., Udhayakumar, Venkatachalam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433255/
https://www.ncbi.nlm.nih.gov/pubmed/25978499
http://dx.doi.org/10.1371/journal.pone.0126805
_version_ 1782371613456465920
author Akinyi Okoth, Sheila
Abdallah, Joseph F.
Ceron, Nicolas
Adhin, Malti R.
Chandrabose, Javin
Krishnalall, Karanchand
Huber, Curtis S.
Goldman, Ira F.
Macedo de Oliveira, Alexandre
Barnwell, John W.
Udhayakumar, Venkatachalam
author_facet Akinyi Okoth, Sheila
Abdallah, Joseph F.
Ceron, Nicolas
Adhin, Malti R.
Chandrabose, Javin
Krishnalall, Karanchand
Huber, Curtis S.
Goldman, Ira F.
Macedo de Oliveira, Alexandre
Barnwell, John W.
Udhayakumar, Venkatachalam
author_sort Akinyi Okoth, Sheila
collection PubMed
description Guyana and Suriname have made important progress in reducing the burden of malaria. While both countries use microscopy as the primary tool for clinical diagnosis, malaria rapid diagnostic tests (RDTs) are useful in remote areas of the interior where laboratory support may be limited or unavailable. Recent reports indicate that histidine-rich protein 2 (PfHRP2)-based diagnostic tests specific for detection of P. falciparum may provide false negative results in some parts of South America due to the emergence of P. falciparum parasites that lack the pfhrp2 gene, and thus produce no PfHRP2 antigen. Pfhrp2 and pfhrp3 genes were amplified in parasite isolates collected from Guyana and Suriname to determine if there were circulating isolates with deletions in these genes. Pfhrp3 deletions were monitored because some monoclonal antibodies utilized in PfHRP2-based RDTs cross-react with the PfHRP3 protein. We found that all 97 isolates from Guyana that met the inclusion criteria were both pfhrp2- and pfhrp3-positive. In Suriname (N = 78), 14% of the samples tested were pfhrp2-negative while 4% were pfhrp3-negative. Furthermore, analysis of the genomic region proximal to pfhrp2 and pfhrp3 revealed that genomic deletions extended to the flanking genes. We also investigated the population substructure of the isolates collected to determine if the parasites that had deletions of pfhrp2 and pfhrp3 belonged to any genetic subtypes. Cluster analysis revealed that there was no predominant P. falciparum population substructure among the isolates from either country, an indication of genetic admixture among the parasite populations. Furthermore, the pfhrp2-deleted parasites from Suriname did not appear to share a single, unique genetic background.
format Online
Article
Text
id pubmed-4433255
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44332552015-05-27 Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname Akinyi Okoth, Sheila Abdallah, Joseph F. Ceron, Nicolas Adhin, Malti R. Chandrabose, Javin Krishnalall, Karanchand Huber, Curtis S. Goldman, Ira F. Macedo de Oliveira, Alexandre Barnwell, John W. Udhayakumar, Venkatachalam PLoS One Research Article Guyana and Suriname have made important progress in reducing the burden of malaria. While both countries use microscopy as the primary tool for clinical diagnosis, malaria rapid diagnostic tests (RDTs) are useful in remote areas of the interior where laboratory support may be limited or unavailable. Recent reports indicate that histidine-rich protein 2 (PfHRP2)-based diagnostic tests specific for detection of P. falciparum may provide false negative results in some parts of South America due to the emergence of P. falciparum parasites that lack the pfhrp2 gene, and thus produce no PfHRP2 antigen. Pfhrp2 and pfhrp3 genes were amplified in parasite isolates collected from Guyana and Suriname to determine if there were circulating isolates with deletions in these genes. Pfhrp3 deletions were monitored because some monoclonal antibodies utilized in PfHRP2-based RDTs cross-react with the PfHRP3 protein. We found that all 97 isolates from Guyana that met the inclusion criteria were both pfhrp2- and pfhrp3-positive. In Suriname (N = 78), 14% of the samples tested were pfhrp2-negative while 4% were pfhrp3-negative. Furthermore, analysis of the genomic region proximal to pfhrp2 and pfhrp3 revealed that genomic deletions extended to the flanking genes. We also investigated the population substructure of the isolates collected to determine if the parasites that had deletions of pfhrp2 and pfhrp3 belonged to any genetic subtypes. Cluster analysis revealed that there was no predominant P. falciparum population substructure among the isolates from either country, an indication of genetic admixture among the parasite populations. Furthermore, the pfhrp2-deleted parasites from Suriname did not appear to share a single, unique genetic background. Public Library of Science 2015-05-15 /pmc/articles/PMC4433255/ /pubmed/25978499 http://dx.doi.org/10.1371/journal.pone.0126805 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Akinyi Okoth, Sheila
Abdallah, Joseph F.
Ceron, Nicolas
Adhin, Malti R.
Chandrabose, Javin
Krishnalall, Karanchand
Huber, Curtis S.
Goldman, Ira F.
Macedo de Oliveira, Alexandre
Barnwell, John W.
Udhayakumar, Venkatachalam
Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname
title Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname
title_full Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname
title_fullStr Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname
title_full_unstemmed Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname
title_short Variation in Plasmodium falciparum Histidine-Rich Protein 2 (Pfhrp2) and Plasmodium falciparum Histidine-Rich Protein 3 (Pfhrp3) Gene Deletions in Guyana and Suriname
title_sort variation in plasmodium falciparum histidine-rich protein 2 (pfhrp2) and plasmodium falciparum histidine-rich protein 3 (pfhrp3) gene deletions in guyana and suriname
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433255/
https://www.ncbi.nlm.nih.gov/pubmed/25978499
http://dx.doi.org/10.1371/journal.pone.0126805
work_keys_str_mv AT akinyiokothsheila variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT abdallahjosephf variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT ceronnicolas variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT adhinmaltir variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT chandrabosejavin variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT krishnalallkaranchand variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT hubercurtiss variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT goldmaniraf variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT macedodeoliveiraalexandre variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT barnwelljohnw variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname
AT udhayakumarvenkatachalam variationinplasmodiumfalciparumhistidinerichprotein2pfhrp2andplasmodiumfalciparumhistidinerichprotein3pfhrp3genedeletionsinguyanaandsuriname