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Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots
An Agrobacterium tumefaciens-mediated corn transformation method based on multiple shoot tissue cultures was developed, which is effective with a variety of corn inbred lines and standard binary vectors. Six factors that affected the success of corn transformation were tested, including A. tumefacie...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433900/ https://www.ncbi.nlm.nih.gov/pubmed/26019506 http://dx.doi.org/10.1080/13102818.2014.907654 |
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author | Cao, Shi-liang Masilamany, Pathmalojiny Li, Wen-bin Pauls, K. Peter |
author_facet | Cao, Shi-liang Masilamany, Pathmalojiny Li, Wen-bin Pauls, K. Peter |
author_sort | Cao, Shi-liang |
collection | PubMed |
description | An Agrobacterium tumefaciens-mediated corn transformation method based on multiple shoot tissue cultures was developed, which is effective with a variety of corn inbred lines and standard binary vectors. Six factors that affected the success of corn transformation were tested, including A. tumefaciens strain, corn genotype, tissue culture growth stage, medium composition, co-culture temperature and surfactant treatment. Agropine-type bacteria (EHA 101 and AGL 1) were eightfold more effective than octopine-type strain for corn multi-shoot tissues transformation. The average frequency of Glucuronidase (GUS)-positive explants obtained from 14 corn genotypes ranged from 36% to 76%. L-proline (0.7 g L(−1)) in the co-culture medium apparently improved the frequency of transformation. The newly initiated multi-shoot tissues were most responsive to Agrobacterium infection. A positive correlation was found between multi-shoot tissue susceptibility to Agrobacterium and the proportion of cells in G1 phase. Transformants were identified by reverse transcription Polymerase Chain Reaction (PCR) and by southern blot hybridization assays. The frequency of transformants was approximately 2% based on the number of multi-shoot explants co-cultivated with Agrobacterium. |
format | Online Article Text |
id | pubmed-4433900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-44339002015-05-25 Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots Cao, Shi-liang Masilamany, Pathmalojiny Li, Wen-bin Pauls, K. Peter Biotechnol Biotechnol Equip Article; Agriculture and Environmental Biotechnology An Agrobacterium tumefaciens-mediated corn transformation method based on multiple shoot tissue cultures was developed, which is effective with a variety of corn inbred lines and standard binary vectors. Six factors that affected the success of corn transformation were tested, including A. tumefaciens strain, corn genotype, tissue culture growth stage, medium composition, co-culture temperature and surfactant treatment. Agropine-type bacteria (EHA 101 and AGL 1) were eightfold more effective than octopine-type strain for corn multi-shoot tissues transformation. The average frequency of Glucuronidase (GUS)-positive explants obtained from 14 corn genotypes ranged from 36% to 76%. L-proline (0.7 g L(−1)) in the co-culture medium apparently improved the frequency of transformation. The newly initiated multi-shoot tissues were most responsive to Agrobacterium infection. A positive correlation was found between multi-shoot tissue susceptibility to Agrobacterium and the proportion of cells in G1 phase. Transformants were identified by reverse transcription Polymerase Chain Reaction (PCR) and by southern blot hybridization assays. The frequency of transformants was approximately 2% based on the number of multi-shoot explants co-cultivated with Agrobacterium. Taylor & Francis 2014-03-04 2014-07-10 /pmc/articles/PMC4433900/ /pubmed/26019506 http://dx.doi.org/10.1080/13102818.2014.907654 Text en © 2014 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Article; Agriculture and Environmental Biotechnology Cao, Shi-liang Masilamany, Pathmalojiny Li, Wen-bin Pauls, K. Peter Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots |
title |
Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots |
title_full |
Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots |
title_fullStr |
Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots |
title_full_unstemmed |
Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots |
title_short |
Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots |
title_sort | agrobacterium tumefaciens-mediated transformation of corn (zea mays l.) multiple shoots |
topic | Article; Agriculture and Environmental Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433900/ https://www.ncbi.nlm.nih.gov/pubmed/26019506 http://dx.doi.org/10.1080/13102818.2014.907654 |
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