Production of a new non-specific nuclease from Yersinia enterocolitica subsp. palearctica: optimization of induction conditions using response surface methodology

A new non-specific nuclease from Yersinia enterocolitica subsp. palearctica (Y. NSN) was expressed in Escherichia coli (E. coli) BL 21 Star(TM) (DE3)plysS. Induction conditions, including isopropyl-β-D-thiogalactoside (IPTG) concentration, cell density (OD600), induction time and induction temperatu...

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Detalles Bibliográficos
Autores principales: Fang, Xiu-Juan, Tang, Zhen-Xing, Li, Zhen-Hua, Zhang, Zhi-Liang, Shi, Lu-E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Articles; Pharmaceutical Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433914/
https://www.ncbi.nlm.nih.gov/pubmed/26019543
http://dx.doi.org/10.1080/13102818.2014.915612

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433914/
https://www.ncbi.nlm.nih.gov/pubmed/26019543
http://dx.doi.org/10.1080/13102818.2014.915612

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