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Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433926/ https://www.ncbi.nlm.nih.gov/pubmed/26019590 http://dx.doi.org/10.1080/13102818.2014.952501 |
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author | Wang, Honghui Pan, Jiachuan Wang, Jing Wang, Nan Zhang, Jie Li, Qiang Wang, Dan Zhou, Xiaohua |
author_facet | Wang, Honghui Pan, Jiachuan Wang, Jing Wang, Nan Zhang, Jie Li, Qiang Wang, Dan Zhou, Xiaohua |
author_sort | Wang, Honghui |
collection | PubMed |
description | Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L(−1) total initial sugars, 42.27 g L(−1) MgCO(3) and 17.84 g L(−1) yeast extract. The maximum production of succinic acid was 52.09 ± 0.21 g L(−1) after 84 h with a yield of 0.63 ± 0.03 g g(−1) total sugar, approaching the predicted value (53.18 g L(−1)). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains. |
format | Online Article Text |
id | pubmed-4433926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-44339262015-05-25 Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain Wang, Honghui Pan, Jiachuan Wang, Jing Wang, Nan Zhang, Jie Li, Qiang Wang, Dan Zhou, Xiaohua Biotechnol Biotechnol Equip Review; Agriculture and Environmental Biotechnology Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L(−1) total initial sugars, 42.27 g L(−1) MgCO(3) and 17.84 g L(−1) yeast extract. The maximum production of succinic acid was 52.09 ± 0.21 g L(−1) after 84 h with a yield of 0.63 ± 0.03 g g(−1) total sugar, approaching the predicted value (53.18 g L(−1)). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains. Taylor & Francis 2014-11-02 2014-11-03 /pmc/articles/PMC4433926/ /pubmed/26019590 http://dx.doi.org/10.1080/13102818.2014.952501 Text en © 2014 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Review; Agriculture and Environmental Biotechnology Wang, Honghui Pan, Jiachuan Wang, Jing Wang, Nan Zhang, Jie Li, Qiang Wang, Dan Zhou, Xiaohua Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain |
title | Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain |
title_full | Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain |
title_fullStr | Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain |
title_full_unstemmed | Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain |
title_short | Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain |
title_sort | succinic acid production from xylose mother liquor by recombinant escherichia coli strain |
topic | Review; Agriculture and Environmental Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433926/ https://www.ncbi.nlm.nih.gov/pubmed/26019590 http://dx.doi.org/10.1080/13102818.2014.952501 |
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