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Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain

Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD...

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Autores principales: Wang, Honghui, Pan, Jiachuan, Wang, Jing, Wang, Nan, Zhang, Jie, Li, Qiang, Wang, Dan, Zhou, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433926/
https://www.ncbi.nlm.nih.gov/pubmed/26019590
http://dx.doi.org/10.1080/13102818.2014.952501
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author Wang, Honghui
Pan, Jiachuan
Wang, Jing
Wang, Nan
Zhang, Jie
Li, Qiang
Wang, Dan
Zhou, Xiaohua
author_facet Wang, Honghui
Pan, Jiachuan
Wang, Jing
Wang, Nan
Zhang, Jie
Li, Qiang
Wang, Dan
Zhou, Xiaohua
author_sort Wang, Honghui
collection PubMed
description Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L(−1) total initial sugars, 42.27 g L(−1) MgCO(3) and 17.84 g L(−1) yeast extract. The maximum production of succinic acid was 52.09 ± 0.21 g L(−1) after 84 h with a yield of 0.63 ± 0.03 g g(−1) total sugar, approaching the predicted value (53.18 g L(−1)). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains.
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spelling pubmed-44339262015-05-25 Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain Wang, Honghui Pan, Jiachuan Wang, Jing Wang, Nan Zhang, Jie Li, Qiang Wang, Dan Zhou, Xiaohua Biotechnol Biotechnol Equip Review; Agriculture and Environmental Biotechnology Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L(−1) total initial sugars, 42.27 g L(−1) MgCO(3) and 17.84 g L(−1) yeast extract. The maximum production of succinic acid was 52.09 ± 0.21 g L(−1) after 84 h with a yield of 0.63 ± 0.03 g g(−1) total sugar, approaching the predicted value (53.18 g L(−1)). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains. Taylor & Francis 2014-11-02 2014-11-03 /pmc/articles/PMC4433926/ /pubmed/26019590 http://dx.doi.org/10.1080/13102818.2014.952501 Text en © 2014 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Review; Agriculture and Environmental Biotechnology
Wang, Honghui
Pan, Jiachuan
Wang, Jing
Wang, Nan
Zhang, Jie
Li, Qiang
Wang, Dan
Zhou, Xiaohua
Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
title Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
title_full Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
title_fullStr Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
title_full_unstemmed Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
title_short Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
title_sort succinic acid production from xylose mother liquor by recombinant escherichia coli strain
topic Review; Agriculture and Environmental Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433926/
https://www.ncbi.nlm.nih.gov/pubmed/26019590
http://dx.doi.org/10.1080/13102818.2014.952501
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