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Identification of pork in meat products using real-time loop-mediated isothermal amplification
In this study, a one-step, real-time, loop-mediated isothermal amplification (RealAmp) assay was developed, for the highly specific detection of pork DNA. For the assay, the mtDNA of cytochrome b (cytb) gene was amplified at 63 °C using SYBR Green I for 45 min with a Real-Time Polymerase Chain React...
Autores principales: | Yang, Lixia, Fu, Shujun, Peng, Xinkai, Li, Le, Song, Taoping, Li, Lin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433938/ https://www.ncbi.nlm.nih.gov/pubmed/26019573 http://dx.doi.org/10.1080/13102818.2014.963789 |
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