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Biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China

A rod-shaped bacterium was isolated from the soil in a karst area of Guilin, China and its biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin was presented for the first time. A total of 22 strains for the degradation of steroidal saponins in sisal were isolated f...

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Detalles Bibliográficos
Autores principales: Wang, Yanchao, Li, Xia, Sun, Hao, Yi, Kexian, Zheng, Jinlong, Zhang, Jie, Hao, Zaibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434041/
https://www.ncbi.nlm.nih.gov/pubmed/26019589
http://dx.doi.org/10.1080/13102818.2014.978199
Descripción
Sumario:A rod-shaped bacterium was isolated from the soil in a karst area of Guilin, China and its biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin was presented for the first time. A total of 22 strains for the degradation of steroidal saponins in sisal were isolated from 48 soil samples, and the isolated rod-shaped, bacterial strain ZG-21 was used for the production of tigogenin due to its highest degradation efficiency of steroidal saponins in sisal. The parameters affecting biotransformation by strain ZG-21 were optimized. Under the optimized conditions of temperature (30 °C), pH (6), time (5 days) and substrate concentration (5 mg/mL), a maximum tigogenin yield of 26.7 mg/g was achieved. Compared with the conventional method of acid hydrolysis, the biotransformation method provided a clean and eco-friendly alternative for the production of tigogenin.