RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape

Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistanc...

Descripción completa

Detalles Bibliográficos
Autores principales: Kord, Hadis, Shakib, Ali Mohammad, Daneshvar, Mohammad Hossein, Azadi, Pejman, Bayat, Vahid, Mashayekhi, Mohsen, Zarea, Mahboobeh, Seifi, Alireza, Ahmad-Raji, Mana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434419/
https://www.ncbi.nlm.nih.gov/pubmed/28324292
http://dx.doi.org/10.1007/s13205-014-0226-9
_version_ 1782371760656613376
author Kord, Hadis
Shakib, Ali Mohammad
Daneshvar, Mohammad Hossein
Azadi, Pejman
Bayat, Vahid
Mashayekhi, Mohsen
Zarea, Mahboobeh
Seifi, Alireza
Ahmad-Raji, Mana
author_facet Kord, Hadis
Shakib, Ali Mohammad
Daneshvar, Mohammad Hossein
Azadi, Pejman
Bayat, Vahid
Mashayekhi, Mohsen
Zarea, Mahboobeh
Seifi, Alireza
Ahmad-Raji, Mana
author_sort Kord, Hadis
collection PubMed
description Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening.
format Online
Article
Text
id pubmed-4434419
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-44344192015-05-19 RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape Kord, Hadis Shakib, Ali Mohammad Daneshvar, Mohammad Hossein Azadi, Pejman Bayat, Vahid Mashayekhi, Mohsen Zarea, Mahboobeh Seifi, Alireza Ahmad-Raji, Mana 3 Biotech Original Article Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening. Springer Berlin Heidelberg 2014-05-22 2015-06 /pmc/articles/PMC4434419/ /pubmed/28324292 http://dx.doi.org/10.1007/s13205-014-0226-9 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/This article is published under license to BioMed Central Ltd.Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Kord, Hadis
Shakib, Ali Mohammad
Daneshvar, Mohammad Hossein
Azadi, Pejman
Bayat, Vahid
Mashayekhi, Mohsen
Zarea, Mahboobeh
Seifi, Alireza
Ahmad-Raji, Mana
RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
title RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
title_full RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
title_fullStr RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
title_full_unstemmed RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
title_short RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
title_sort rnai-mediated down-regulation of shatterproof gene in transgenic oilseed rape
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434419/
https://www.ncbi.nlm.nih.gov/pubmed/28324292
http://dx.doi.org/10.1007/s13205-014-0226-9
work_keys_str_mv AT kordhadis rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT shakibalimohammad rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT daneshvarmohammadhossein rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT azadipejman rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT bayatvahid rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT mashayekhimohsen rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT zareamahboobeh rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT seifialireza rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape
AT ahmadrajimana rnaimediateddownregulationofshatterproofgeneintransgenicoilseedrape

Ejemplares similares