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Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine

Convolvulus pluricaulis Choisy, commonly known as “Shankhpushpi”, is an ayurvedic medicinal plant recommended as a brain tonic to promote intellect and memory, eliminate nervous disorders and to treat hypertension. Because of increasing demand of the drug, this plant species has been over-exploited....

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Autores principales: Ganie, Showkat Hussain, Ali, Zahid, Das, Sandip, Srivastava, Prem Shankar, Sharma, Maheshwar Prasad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434420/
https://www.ncbi.nlm.nih.gov/pubmed/28324296
http://dx.doi.org/10.1007/s13205-014-0227-8
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author Ganie, Showkat Hussain
Ali, Zahid
Das, Sandip
Srivastava, Prem Shankar
Sharma, Maheshwar Prasad
author_facet Ganie, Showkat Hussain
Ali, Zahid
Das, Sandip
Srivastava, Prem Shankar
Sharma, Maheshwar Prasad
author_sort Ganie, Showkat Hussain
collection PubMed
description Convolvulus pluricaulis Choisy, commonly known as “Shankhpushpi”, is an ayurvedic medicinal plant recommended as a brain tonic to promote intellect and memory, eliminate nervous disorders and to treat hypertension. Because of increasing demand of the drug, this plant species has been over-exploited. As a consequence, many unrelated plants are being sold by the crude drug dealers in India in the name of “Shankhpushpi”. Information on its existing gene pool is currently lacking. We developed molecular (Random Amplification of Polymorphic DNA) and chemical (high performance liquid chromatography) markers that could distinguish the genuine plant species from its adulterants. Molecular characterization confirmed higher genetic variation at inter-zonal level as compared to intra-zonal populations. A total of 37 reproducible amplicons were generated of which 22 were polymorphic. The number of amplicons was in the range of 6–11 and genetic distance for the studied primers ranged from 0.07 to 0.34. Fifty nine per cent polymorphism was obtained across different geographical locations. Dendrogram studied through unweighted pair group method of arithmetic analysis differentiated all the genotypes into two major clusters, Cluster I had the single population of Rajasthan and Cluster II was represented by genotypes of Delhi, Haryana, Madhya Pradesh and Rajasthan. The Kaempferol content ranged from 0.07 to 0.49 mg/g and Delhi population was the highest accumulator.
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spelling pubmed-44344202015-05-19 Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine Ganie, Showkat Hussain Ali, Zahid Das, Sandip Srivastava, Prem Shankar Sharma, Maheshwar Prasad 3 Biotech Original Article Convolvulus pluricaulis Choisy, commonly known as “Shankhpushpi”, is an ayurvedic medicinal plant recommended as a brain tonic to promote intellect and memory, eliminate nervous disorders and to treat hypertension. Because of increasing demand of the drug, this plant species has been over-exploited. As a consequence, many unrelated plants are being sold by the crude drug dealers in India in the name of “Shankhpushpi”. Information on its existing gene pool is currently lacking. We developed molecular (Random Amplification of Polymorphic DNA) and chemical (high performance liquid chromatography) markers that could distinguish the genuine plant species from its adulterants. Molecular characterization confirmed higher genetic variation at inter-zonal level as compared to intra-zonal populations. A total of 37 reproducible amplicons were generated of which 22 were polymorphic. The number of amplicons was in the range of 6–11 and genetic distance for the studied primers ranged from 0.07 to 0.34. Fifty nine per cent polymorphism was obtained across different geographical locations. Dendrogram studied through unweighted pair group method of arithmetic analysis differentiated all the genotypes into two major clusters, Cluster I had the single population of Rajasthan and Cluster II was represented by genotypes of Delhi, Haryana, Madhya Pradesh and Rajasthan. The Kaempferol content ranged from 0.07 to 0.49 mg/g and Delhi population was the highest accumulator. Springer Berlin Heidelberg 2014-06-18 2015-06 /pmc/articles/PMC4434420/ /pubmed/28324296 http://dx.doi.org/10.1007/s13205-014-0227-8 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/This article is published under license to BioMed Central Ltd.Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Ganie, Showkat Hussain
Ali, Zahid
Das, Sandip
Srivastava, Prem Shankar
Sharma, Maheshwar Prasad
Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
title Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
title_full Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
title_fullStr Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
title_full_unstemmed Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
title_short Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
title_sort genetic diversity and chemical profiling of different populations of convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434420/
https://www.ncbi.nlm.nih.gov/pubmed/28324296
http://dx.doi.org/10.1007/s13205-014-0227-8
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