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Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways
Apolipoprotein CIII (apo CIII), a small glycoprotein that binds to the surfaces of certain lipoproteins, is associated with inflammatory and atherogenic responses in vascular cells. Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) has been proposed as an inflammatory biomarker and potential the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434817/ https://www.ncbi.nlm.nih.gov/pubmed/25836672 http://dx.doi.org/10.1242/bio.201410900 |
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author | Han, Xiaolei Wang, Tiedong Zhang, Jifeng Liu, Xingxing Li, Zhuang Wang, Gangqi Song, Qi Pang, Daxin Ouyang, Hongsheng Tang, Xiaochun |
author_facet | Han, Xiaolei Wang, Tiedong Zhang, Jifeng Liu, Xingxing Li, Zhuang Wang, Gangqi Song, Qi Pang, Daxin Ouyang, Hongsheng Tang, Xiaochun |
author_sort | Han, Xiaolei |
collection | PubMed |
description | Apolipoprotein CIII (apo CIII), a small glycoprotein that binds to the surfaces of certain lipoproteins, is associated with inflammatory and atherogenic responses in vascular cells. Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) has been proposed as an inflammatory biomarker and potential therapeutic target for cardiovascular disease (CVD). Here, we report that apo CIII increases Lp-PLA(2) mRNA and protein levels in dose- and time- dependent manner in human monocytic THP-1 cells, and the increase can be abolished by MAPK and NFκB pathway inhibitors. Lp-PLA(2) inhibitor, 1-linoleoyl glycerol attenuates the inflammation induced by apo CIII. In turn, exogenous Lp-PLA(2) expression upregulates apo CIII and the upregulation can be inhibited by 1-linoleoyl glycerol in HepG2 cells. Moreover, plasma Lp-PLA(2) level is correlated with apo CIII expression in pig liver. In vivo, Lp-PLA(2) expression in monocytes and its activity in serum were significantly increased in human apo CIII transgenic porcine models compared with wild-type pigs. Our results suggest that Lp-PLA(2) and apo CIII expression level is correlated with each other in vitro and in vivo. |
format | Online Article Text |
id | pubmed-4434817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-44348172015-06-11 Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways Han, Xiaolei Wang, Tiedong Zhang, Jifeng Liu, Xingxing Li, Zhuang Wang, Gangqi Song, Qi Pang, Daxin Ouyang, Hongsheng Tang, Xiaochun Biol Open Research Article Apolipoprotein CIII (apo CIII), a small glycoprotein that binds to the surfaces of certain lipoproteins, is associated with inflammatory and atherogenic responses in vascular cells. Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) has been proposed as an inflammatory biomarker and potential therapeutic target for cardiovascular disease (CVD). Here, we report that apo CIII increases Lp-PLA(2) mRNA and protein levels in dose- and time- dependent manner in human monocytic THP-1 cells, and the increase can be abolished by MAPK and NFκB pathway inhibitors. Lp-PLA(2) inhibitor, 1-linoleoyl glycerol attenuates the inflammation induced by apo CIII. In turn, exogenous Lp-PLA(2) expression upregulates apo CIII and the upregulation can be inhibited by 1-linoleoyl glycerol in HepG2 cells. Moreover, plasma Lp-PLA(2) level is correlated with apo CIII expression in pig liver. In vivo, Lp-PLA(2) expression in monocytes and its activity in serum were significantly increased in human apo CIII transgenic porcine models compared with wild-type pigs. Our results suggest that Lp-PLA(2) and apo CIII expression level is correlated with each other in vitro and in vivo. The Company of Biologists 2015-04-02 /pmc/articles/PMC4434817/ /pubmed/25836672 http://dx.doi.org/10.1242/bio.201410900 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Han, Xiaolei Wang, Tiedong Zhang, Jifeng Liu, Xingxing Li, Zhuang Wang, Gangqi Song, Qi Pang, Daxin Ouyang, Hongsheng Tang, Xiaochun Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways |
title | Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways |
title_full | Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways |
title_fullStr | Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways |
title_full_unstemmed | Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways |
title_short | Apolipoprotein CIII regulates lipoprotein-associated phospholipase A(2) expression via the MAPK and NFκB pathways |
title_sort | apolipoprotein ciii regulates lipoprotein-associated phospholipase a(2) expression via the mapk and nfκb pathways |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434817/ https://www.ncbi.nlm.nih.gov/pubmed/25836672 http://dx.doi.org/10.1242/bio.201410900 |
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