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Activity-dependent plasticity of mouse hippocampal assemblies in vitro
Memory formation is associated with the generation of transiently stable neuronal assemblies. In hippocampal networks, such groups of functionally coupled neurons express highly ordered spatiotemporal activity patterns which are coordinated by local network oscillations. One of these patterns, sharp...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435105/ https://www.ncbi.nlm.nih.gov/pubmed/26041998 http://dx.doi.org/10.3389/fncir.2015.00021 |
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author | Keller, Martin K. Draguhn, Andreas Both, Martin Reichinnek, Susanne |
author_facet | Keller, Martin K. Draguhn, Andreas Both, Martin Reichinnek, Susanne |
author_sort | Keller, Martin K. |
collection | PubMed |
description | Memory formation is associated with the generation of transiently stable neuronal assemblies. In hippocampal networks, such groups of functionally coupled neurons express highly ordered spatiotemporal activity patterns which are coordinated by local network oscillations. One of these patterns, sharp wave-ripple complexes (SPW-R), repetitively activates previously established groups of memory-encoding neurons, thereby supporting memory consolidation. This function implies that repetition of specific SPW-R induces plastic changes which render the underlying neuronal assemblies more stable. We modeled this repetitive activation in an in vitro model of SPW-R in mouse hippocampal slices. Weak electrical stimulation upstream of the CA3-CA1 networks reliably induced SPW-R of stereotypic waveform, thus representing re-activation of similar neuronal activity patterns. Frequent repetition of these patterns (100 times) reduced the variance of both, evoked and spontaneous SPW-R waveforms, indicating stabilization of pre-existing assemblies. These effects were most pronounced in the CA1 subfield and depended on the timing of stimulation relative to spontaneous SPW-R. Additionally, plasticity of SPW-R was blocked by application of a NMDA receptor antagonist, suggesting a role for associative synaptic plasticity in this process. Thus, repetitive activation of specific patterns of SPW-R causes stabilization of memory-related networks. |
format | Online Article Text |
id | pubmed-4435105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44351052015-06-03 Activity-dependent plasticity of mouse hippocampal assemblies in vitro Keller, Martin K. Draguhn, Andreas Both, Martin Reichinnek, Susanne Front Neural Circuits Neuroscience Memory formation is associated with the generation of transiently stable neuronal assemblies. In hippocampal networks, such groups of functionally coupled neurons express highly ordered spatiotemporal activity patterns which are coordinated by local network oscillations. One of these patterns, sharp wave-ripple complexes (SPW-R), repetitively activates previously established groups of memory-encoding neurons, thereby supporting memory consolidation. This function implies that repetition of specific SPW-R induces plastic changes which render the underlying neuronal assemblies more stable. We modeled this repetitive activation in an in vitro model of SPW-R in mouse hippocampal slices. Weak electrical stimulation upstream of the CA3-CA1 networks reliably induced SPW-R of stereotypic waveform, thus representing re-activation of similar neuronal activity patterns. Frequent repetition of these patterns (100 times) reduced the variance of both, evoked and spontaneous SPW-R waveforms, indicating stabilization of pre-existing assemblies. These effects were most pronounced in the CA1 subfield and depended on the timing of stimulation relative to spontaneous SPW-R. Additionally, plasticity of SPW-R was blocked by application of a NMDA receptor antagonist, suggesting a role for associative synaptic plasticity in this process. Thus, repetitive activation of specific patterns of SPW-R causes stabilization of memory-related networks. Frontiers Media S.A. 2015-05-18 /pmc/articles/PMC4435105/ /pubmed/26041998 http://dx.doi.org/10.3389/fncir.2015.00021 Text en Copyright © 2015 Keller, Draguhn, Both and Reichinnek. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Keller, Martin K. Draguhn, Andreas Both, Martin Reichinnek, Susanne Activity-dependent plasticity of mouse hippocampal assemblies in vitro |
title | Activity-dependent plasticity of mouse hippocampal assemblies in vitro |
title_full | Activity-dependent plasticity of mouse hippocampal assemblies in vitro |
title_fullStr | Activity-dependent plasticity of mouse hippocampal assemblies in vitro |
title_full_unstemmed | Activity-dependent plasticity of mouse hippocampal assemblies in vitro |
title_short | Activity-dependent plasticity of mouse hippocampal assemblies in vitro |
title_sort | activity-dependent plasticity of mouse hippocampal assemblies in vitro |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435105/ https://www.ncbi.nlm.nih.gov/pubmed/26041998 http://dx.doi.org/10.3389/fncir.2015.00021 |
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