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Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform

In this paper, we propose an easy-to-implement passive liquid valve (PLV) for the microfluidic compact-disc (CD). This valve can be implemented by introducing venting chambers to control the air flow of the source and destination chambers. The PLV mechanism is based on equalizing the main forces act...

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Autores principales: Al-Faqheri, Wisam, Ibrahim, Fatimah, Thio, Tzer Hwai Gilbert, Bahari, Norulain, Arof, Hamzah, Rothan, Hussin A., Yusof, Rohana, Madou, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435176/
https://www.ncbi.nlm.nih.gov/pubmed/25723143
http://dx.doi.org/10.3390/s150304658
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author Al-Faqheri, Wisam
Ibrahim, Fatimah
Thio, Tzer Hwai Gilbert
Bahari, Norulain
Arof, Hamzah
Rothan, Hussin A.
Yusof, Rohana
Madou, Marc
author_facet Al-Faqheri, Wisam
Ibrahim, Fatimah
Thio, Tzer Hwai Gilbert
Bahari, Norulain
Arof, Hamzah
Rothan, Hussin A.
Yusof, Rohana
Madou, Marc
author_sort Al-Faqheri, Wisam
collection PubMed
description In this paper, we propose an easy-to-implement passive liquid valve (PLV) for the microfluidic compact-disc (CD). This valve can be implemented by introducing venting chambers to control the air flow of the source and destination chambers. The PLV mechanism is based on equalizing the main forces acting on the microfluidic CD (i.e., the centrifugal and capillary forces) to control the burst frequency of the source chamber liquid. For a better understanding of the physics behind the proposed PLV, an analytical model is described. Moreover, three parameters that control the effectiveness of the proposed valve, i.e., the liquid height, liquid density, and venting chamber position with respect to the CD center, are tested experimentally. To demonstrate the ability of the proposed PLV valve, microfluidic liquid switching and liquid metering are performed. In addition, a Bradford assay is performed to measure the protein concentration and evaluated in comparison to the benchtop procedure. The result shows that the proposed valve can be implemented in any microfluidic process that requires simplicity and accuracy. Moreover, the developed valve increases the flexibility of the centrifugal CD platform for passive control of the liquid flow without the need for an external force or trigger.
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spelling pubmed-44351762015-05-19 Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform Al-Faqheri, Wisam Ibrahim, Fatimah Thio, Tzer Hwai Gilbert Bahari, Norulain Arof, Hamzah Rothan, Hussin A. Yusof, Rohana Madou, Marc Sensors (Basel) Article In this paper, we propose an easy-to-implement passive liquid valve (PLV) for the microfluidic compact-disc (CD). This valve can be implemented by introducing venting chambers to control the air flow of the source and destination chambers. The PLV mechanism is based on equalizing the main forces acting on the microfluidic CD (i.e., the centrifugal and capillary forces) to control the burst frequency of the source chamber liquid. For a better understanding of the physics behind the proposed PLV, an analytical model is described. Moreover, three parameters that control the effectiveness of the proposed valve, i.e., the liquid height, liquid density, and venting chamber position with respect to the CD center, are tested experimentally. To demonstrate the ability of the proposed PLV valve, microfluidic liquid switching and liquid metering are performed. In addition, a Bradford assay is performed to measure the protein concentration and evaluated in comparison to the benchtop procedure. The result shows that the proposed valve can be implemented in any microfluidic process that requires simplicity and accuracy. Moreover, the developed valve increases the flexibility of the centrifugal CD platform for passive control of the liquid flow without the need for an external force or trigger. MDPI 2015-02-25 /pmc/articles/PMC4435176/ /pubmed/25723143 http://dx.doi.org/10.3390/s150304658 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Faqheri, Wisam
Ibrahim, Fatimah
Thio, Tzer Hwai Gilbert
Bahari, Norulain
Arof, Hamzah
Rothan, Hussin A.
Yusof, Rohana
Madou, Marc
Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
title Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
title_full Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
title_fullStr Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
title_full_unstemmed Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
title_short Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
title_sort development of a passive liquid valve (plv) utilizing a pressure equilibrium phenomenon on the centrifugal microfluidic platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435176/
https://www.ncbi.nlm.nih.gov/pubmed/25723143
http://dx.doi.org/10.3390/s150304658
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