Cargando…
Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip
We report an ultra-sensitive and robust fluorescence sensor made by using a biconical taper with a waist diameter of 720 nm for both excitation and fluorescence collection. To enhance the stability of the fluorescence sensor, the biconical taper has been embedded in a 125 µm wide microchannel with a...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435202/ https://www.ncbi.nlm.nih.gov/pubmed/25808762 http://dx.doi.org/10.3390/s150304890 |
_version_ | 1782371873845149696 |
---|---|
author | Li, Zhiyong Xu, Yingxin Fang, Wei Tong, Limin Zhang, Lei |
author_facet | Li, Zhiyong Xu, Yingxin Fang, Wei Tong, Limin Zhang, Lei |
author_sort | Li, Zhiyong |
collection | PubMed |
description | We report an ultra-sensitive and robust fluorescence sensor made by using a biconical taper with a waist diameter of 720 nm for both excitation and fluorescence collection. To enhance the stability of the fluorescence sensor, the biconical taper has been embedded in a 125 µm wide microchannel with a detection length of 2.5 cm. Investigated by measuring the fluorescence intensity of rhodamine 6G (R6G), the sensor shows a detection limit down to 100 pM, with excellent reversibility in a concentration range of 0–10 nM. The sensor has also been applied to quantum dot (QD)-labeled streptavidin measurements, yielding a detection sensitivity down to 10 pM for QDs. In addition, the small sample volume (ca. 500 nL), high sampling throughput, and seamless connection between the biconical taper and standard optical fibers offer a number of attractive advantages for chemical and biosensing applications. |
format | Online Article Text |
id | pubmed-4435202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44352022015-05-19 Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip Li, Zhiyong Xu, Yingxin Fang, Wei Tong, Limin Zhang, Lei Sensors (Basel) Communication We report an ultra-sensitive and robust fluorescence sensor made by using a biconical taper with a waist diameter of 720 nm for both excitation and fluorescence collection. To enhance the stability of the fluorescence sensor, the biconical taper has been embedded in a 125 µm wide microchannel with a detection length of 2.5 cm. Investigated by measuring the fluorescence intensity of rhodamine 6G (R6G), the sensor shows a detection limit down to 100 pM, with excellent reversibility in a concentration range of 0–10 nM. The sensor has also been applied to quantum dot (QD)-labeled streptavidin measurements, yielding a detection sensitivity down to 10 pM for QDs. In addition, the small sample volume (ca. 500 nL), high sampling throughput, and seamless connection between the biconical taper and standard optical fibers offer a number of attractive advantages for chemical and biosensing applications. MDPI 2015-02-26 /pmc/articles/PMC4435202/ /pubmed/25808762 http://dx.doi.org/10.3390/s150304890 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Li, Zhiyong Xu, Yingxin Fang, Wei Tong, Limin Zhang, Lei Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
title | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
title_full | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
title_fullStr | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
title_full_unstemmed | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
title_short | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
title_sort | ultra-sensitive nanofiber fluorescence detection in a microfluidic chip |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435202/ https://www.ncbi.nlm.nih.gov/pubmed/25808762 http://dx.doi.org/10.3390/s150304890 |
work_keys_str_mv | AT lizhiyong ultrasensitivenanofiberfluorescencedetectioninamicrofluidicchip AT xuyingxin ultrasensitivenanofiberfluorescencedetectioninamicrofluidicchip AT fangwei ultrasensitivenanofiberfluorescencedetectioninamicrofluidicchip AT tonglimin ultrasensitivenanofiberfluorescencedetectioninamicrofluidicchip AT zhanglei ultrasensitivenanofiberfluorescencedetectioninamicrofluidicchip |