Cargando…
Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices
Integrated microfluidic devices with nanosized array electrodes and microfiltration capabilities can greatly increase sensitivity and enhance automation in immunoassay devices. In this contribution, we utilize the edge-patterning method of thin aluminum (Al) films in order to form nano- to micron-si...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435220/ https://www.ncbi.nlm.nih.gov/pubmed/25774709 http://dx.doi.org/10.3390/s150306091 |
_version_ | 1782371878018482176 |
---|---|
author | Doan, Nhi M. Qiang, Liangliang Li, Zhe Vaddiraju, Santhisagar Bishop, Gregory W. Rusling, James F. Papadimitrakopoulos, Fotios |
author_facet | Doan, Nhi M. Qiang, Liangliang Li, Zhe Vaddiraju, Santhisagar Bishop, Gregory W. Rusling, James F. Papadimitrakopoulos, Fotios |
author_sort | Doan, Nhi M. |
collection | PubMed |
description | Integrated microfluidic devices with nanosized array electrodes and microfiltration capabilities can greatly increase sensitivity and enhance automation in immunoassay devices. In this contribution, we utilize the edge-patterning method of thin aluminum (Al) films in order to form nano- to micron-sized gaps. Evaporation of high work-function metals (i.e., Au, Ag, etc.) on these gaps, followed by Al lift-off, enables the formation of electrical uniform nanowires from low-cost, plastic-based, photomasks. By replacing Al with chromium (Cr), the formation of high resolution, custom-made photomasks that are ideal for low-cost fabrication of a plurality of array devices were realized. To demonstrate the feasibility of such Cr photomasks, SU-8 micro-pillar masters were formed and replicated into PDMS to produce micron-sized filters with 3–4 µm gaps and an aspect ratio of 3. These microfilters were capable of retaining 6 µm beads within a localized site, while allowing solvent flow. The combination of nanowire arrays and micro-pillar filtration opens new perspectives for rapid R&D screening of various microfluidic-based immunoassay geometries, where analyte pre-concentration and highly sensitive, electrochemical detection can be readily co-localized. |
format | Online Article Text |
id | pubmed-4435220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44352202015-05-19 Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices Doan, Nhi M. Qiang, Liangliang Li, Zhe Vaddiraju, Santhisagar Bishop, Gregory W. Rusling, James F. Papadimitrakopoulos, Fotios Sensors (Basel) Article Integrated microfluidic devices with nanosized array electrodes and microfiltration capabilities can greatly increase sensitivity and enhance automation in immunoassay devices. In this contribution, we utilize the edge-patterning method of thin aluminum (Al) films in order to form nano- to micron-sized gaps. Evaporation of high work-function metals (i.e., Au, Ag, etc.) on these gaps, followed by Al lift-off, enables the formation of electrical uniform nanowires from low-cost, plastic-based, photomasks. By replacing Al with chromium (Cr), the formation of high resolution, custom-made photomasks that are ideal for low-cost fabrication of a plurality of array devices were realized. To demonstrate the feasibility of such Cr photomasks, SU-8 micro-pillar masters were formed and replicated into PDMS to produce micron-sized filters with 3–4 µm gaps and an aspect ratio of 3. These microfilters were capable of retaining 6 µm beads within a localized site, while allowing solvent flow. The combination of nanowire arrays and micro-pillar filtration opens new perspectives for rapid R&D screening of various microfluidic-based immunoassay geometries, where analyte pre-concentration and highly sensitive, electrochemical detection can be readily co-localized. MDPI 2015-03-12 /pmc/articles/PMC4435220/ /pubmed/25774709 http://dx.doi.org/10.3390/s150306091 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Doan, Nhi M. Qiang, Liangliang Li, Zhe Vaddiraju, Santhisagar Bishop, Gregory W. Rusling, James F. Papadimitrakopoulos, Fotios Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices |
title | Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices |
title_full | Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices |
title_fullStr | Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices |
title_full_unstemmed | Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices |
title_short | Low-Cost Photolithographic Fabrication of Nanowires and Microfilters for Advanced Bioassay Devices |
title_sort | low-cost photolithographic fabrication of nanowires and microfilters for advanced bioassay devices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435220/ https://www.ncbi.nlm.nih.gov/pubmed/25774709 http://dx.doi.org/10.3390/s150306091 |
work_keys_str_mv | AT doannhim lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices AT qiangliangliang lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices AT lizhe lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices AT vaddirajusanthisagar lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices AT bishopgregoryw lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices AT ruslingjamesf lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices AT papadimitrakopoulosfotios lowcostphotolithographicfabricationofnanowiresandmicrofiltersforadvancedbioassaydevices |