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Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)

PREMISE OF THE STUDY: Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity. METHODS AND RESULTS: Eight microsatellite markers wer...

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Autores principales: Celik, Mélina, Wegnez, Jérôme, Griveau, Chantal, Lambourdière, Josie, Utge, Jose, Noël, Florence, Abdelkrim, Jawad, Machon, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435467/
https://www.ncbi.nlm.nih.gov/pubmed/25995977
http://dx.doi.org/10.3732/apps.1500011
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author Celik, Mélina
Wegnez, Jérôme
Griveau, Chantal
Lambourdière, Josie
Utge, Jose
Noël, Florence
Abdelkrim, Jawad
Machon, Nathalie
author_facet Celik, Mélina
Wegnez, Jérôme
Griveau, Chantal
Lambourdière, Josie
Utge, Jose
Noël, Florence
Abdelkrim, Jawad
Machon, Nathalie
author_sort Celik, Mélina
collection PubMed
description PREMISE OF THE STUDY: Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity. METHODS AND RESULTS: Eight microsatellite markers were isolated using next-generation sequencing. Loci were amplified and screened for 138 individuals in 17 populations from France. Two of the eight polymorphic loci presented no variability across populations. The total number of alleles per locus varied from two to four. Observed heterozygosity ranged from 0.051 to 1.000. All primers amplified successfully in the closely related species V. pumila. CONCLUSIONS: This set of microsatellites offers a valuable tool for assessing population genetic diversity of the species to improve its conservation and base management efforts. High observed heterozygosity values probably reflect the particular mating system of the species and suggest an important tendency to clonality.
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spelling pubmed-44354672015-05-20 Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1) Celik, Mélina Wegnez, Jérôme Griveau, Chantal Lambourdière, Josie Utge, Jose Noël, Florence Abdelkrim, Jawad Machon, Nathalie Appl Plant Sci Primer Note PREMISE OF THE STUDY: Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity. METHODS AND RESULTS: Eight microsatellite markers were isolated using next-generation sequencing. Loci were amplified and screened for 138 individuals in 17 populations from France. Two of the eight polymorphic loci presented no variability across populations. The total number of alleles per locus varied from two to four. Observed heterozygosity ranged from 0.051 to 1.000. All primers amplified successfully in the closely related species V. pumila. CONCLUSIONS: This set of microsatellites offers a valuable tool for assessing population genetic diversity of the species to improve its conservation and base management efforts. High observed heterozygosity values probably reflect the particular mating system of the species and suggest an important tendency to clonality. Botanical Society of America 2015-05-13 /pmc/articles/PMC4435467/ /pubmed/25995977 http://dx.doi.org/10.3732/apps.1500011 Text en © 2015 Celik et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Celik, Mélina
Wegnez, Jérôme
Griveau, Chantal
Lambourdière, Josie
Utge, Jose
Noël, Florence
Abdelkrim, Jawad
Machon, Nathalie
Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
title Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
title_full Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
title_fullStr Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
title_full_unstemmed Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
title_short Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
title_sort development of microsatellite primers in the protected species viola elatior (violaceae) using next-generation sequencing(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435467/
https://www.ncbi.nlm.nih.gov/pubmed/25995977
http://dx.doi.org/10.3732/apps.1500011
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