Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)

PREMISE OF THE STUDY: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. METHODS AND RESULTS: Using an enriched lib...

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Detalles Bibliográficos
Autores principales: Letelier, Luis, Harvey, Nick, Valderrama, Aly, Stoll, Alexandra, González-Rodríguez, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435470/
https://www.ncbi.nlm.nih.gov/pubmed/25995980
http://dx.doi.org/10.3732/apps.1500024
Descripción
Sumario:PREMISE OF THE STUDY: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. METHODS AND RESULTS: Using an enriched library, 12 polymorphic microsatellite loci were developed in Q. saponaria. All loci consisted of dinucleotide repeats. The average number of alleles per locus was 5.3 (2–13), with a total of 64 alleles recorded in 39 individuals from three populations. CONCLUSIONS: The microsatellite markers described here are the first characterized for Q. saponaria. The polymorphic loci will be useful in studies of genetic diversity and genetic population differentiation in natural populations of this species.

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