Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)

PREMISE OF THE STUDY: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. METHODS AND RESULTS: Using an enriched lib...

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Detalles Bibliográficos
Autores principales: Letelier, Luis, Harvey, Nick, Valderrama, Aly, Stoll, Alexandra, González-Rodríguez, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435470/
https://www.ncbi.nlm.nih.gov/pubmed/25995980
http://dx.doi.org/10.3732/apps.1500024
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author Letelier, Luis
Harvey, Nick
Valderrama, Aly
Stoll, Alexandra
González-Rodríguez, Antonio
author_facet Letelier, Luis
Harvey, Nick
Valderrama, Aly
Stoll, Alexandra
González-Rodríguez, Antonio
author_sort Letelier, Luis
collection PubMed
description PREMISE OF THE STUDY: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. METHODS AND RESULTS: Using an enriched library, 12 polymorphic microsatellite loci were developed in Q. saponaria. All loci consisted of dinucleotide repeats. The average number of alleles per locus was 5.3 (2–13), with a total of 64 alleles recorded in 39 individuals from three populations. CONCLUSIONS: The microsatellite markers described here are the first characterized for Q. saponaria. The polymorphic loci will be useful in studies of genetic diversity and genetic population differentiation in natural populations of this species.
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spelling pubmed-44354702015-05-20 Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1) Letelier, Luis Harvey, Nick Valderrama, Aly Stoll, Alexandra González-Rodríguez, Antonio Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. METHODS AND RESULTS: Using an enriched library, 12 polymorphic microsatellite loci were developed in Q. saponaria. All loci consisted of dinucleotide repeats. The average number of alleles per locus was 5.3 (2–13), with a total of 64 alleles recorded in 39 individuals from three populations. CONCLUSIONS: The microsatellite markers described here are the first characterized for Q. saponaria. The polymorphic loci will be useful in studies of genetic diversity and genetic population differentiation in natural populations of this species. Botanical Society of America 2015-05-11 /pmc/articles/PMC4435470/ /pubmed/25995980 http://dx.doi.org/10.3732/apps.1500024 Text en © 2015 Letelier et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Letelier, Luis
Harvey, Nick
Valderrama, Aly
Stoll, Alexandra
González-Rodríguez, Antonio
Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)
title Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)
title_full Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)
title_fullStr Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)
title_full_unstemmed Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)
title_short Isolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)(1)
title_sort isolation and characterization of 12 microsatellite loci in soapbark, quillaja saponaria (quillajaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435470/
https://www.ncbi.nlm.nih.gov/pubmed/25995980
http://dx.doi.org/10.3732/apps.1500024
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