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A comparison of high-throughput techniques for assaying circadian rhythms in plants
Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this ha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435651/ https://www.ncbi.nlm.nih.gov/pubmed/25987891 http://dx.doi.org/10.1186/s13007-015-0071-9 |
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author | Tindall, Andrew J Waller, Jade Greenwood, Mark Gould, Peter D Hartwell, James Hall, Anthony |
author_facet | Tindall, Andrew J Waller, Jade Greenwood, Mark Gould, Peter D Hartwell, James Hall, Anthony |
author_sort | Tindall, Andrew J |
collection | PubMed |
description | Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this has opened up new avenues for understanding plant development and signalling. Our tool-kit has been constantly improving through continual development and novel techniques that increase throughput, reduce costs and allow higher resolution on the cellular and subcellular levels. With circadian assays becoming more accessible and relevant than ever to researchers, in this paper we offer a review of the techniques currently available before considering the horizons in circadian investigation at ever higher throughputs and resolutions. |
format | Online Article Text |
id | pubmed-4435651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44356512015-05-19 A comparison of high-throughput techniques for assaying circadian rhythms in plants Tindall, Andrew J Waller, Jade Greenwood, Mark Gould, Peter D Hartwell, James Hall, Anthony Plant Methods Review Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this has opened up new avenues for understanding plant development and signalling. Our tool-kit has been constantly improving through continual development and novel techniques that increase throughput, reduce costs and allow higher resolution on the cellular and subcellular levels. With circadian assays becoming more accessible and relevant than ever to researchers, in this paper we offer a review of the techniques currently available before considering the horizons in circadian investigation at ever higher throughputs and resolutions. BioMed Central 2015-05-03 /pmc/articles/PMC4435651/ /pubmed/25987891 http://dx.doi.org/10.1186/s13007-015-0071-9 Text en © Tindall et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Tindall, Andrew J Waller, Jade Greenwood, Mark Gould, Peter D Hartwell, James Hall, Anthony A comparison of high-throughput techniques for assaying circadian rhythms in plants |
title | A comparison of high-throughput techniques for assaying circadian rhythms in plants |
title_full | A comparison of high-throughput techniques for assaying circadian rhythms in plants |
title_fullStr | A comparison of high-throughput techniques for assaying circadian rhythms in plants |
title_full_unstemmed | A comparison of high-throughput techniques for assaying circadian rhythms in plants |
title_short | A comparison of high-throughput techniques for assaying circadian rhythms in plants |
title_sort | comparison of high-throughput techniques for assaying circadian rhythms in plants |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435651/ https://www.ncbi.nlm.nih.gov/pubmed/25987891 http://dx.doi.org/10.1186/s13007-015-0071-9 |
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