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Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?

[Image: see text] A detailed and systematic polarized confocal fluorescence microscopy investigation is presented on three batches of large coffin-shaped ZSM-5 crystals (i.e., parent, steamed at 500 °C, and steamed at 700 °C). In total, six laser lines of different wavelength in the visible region a...

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Autores principales: Sprung, Christoph, Weckhuysen, Bert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435783/
https://www.ncbi.nlm.nih.gov/pubmed/25590519
http://dx.doi.org/10.1021/ja511381f
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author Sprung, Christoph
Weckhuysen, Bert M.
author_facet Sprung, Christoph
Weckhuysen, Bert M.
author_sort Sprung, Christoph
collection PubMed
description [Image: see text] A detailed and systematic polarized confocal fluorescence microscopy investigation is presented on three batches of large coffin-shaped ZSM-5 crystals (i.e., parent, steamed at 500 °C, and steamed at 700 °C). In total, six laser lines of different wavelength in the visible region are employed on two crystal positions and three orientations with respect to the polarization plane of the excitation laser light. A fluorescent probe molecule is generated inside the zeolite pores, originating from the acid-catalyzed oligomerization of 4-fluorostyrene. A thorough analysis of the polarization plane of emitting fluorescent light reveals insight into the orientation of the fluorescent probe molecule restricted by the highly ordered zeolite channel framework, thereby visualizing pore accessibility and clearly distinguishing the occupation of straight and sinusoidal channels by the probe molecule. Spectral features are, furthermore, observed to tell apart molecules situated in one or the other pore. Special focus was given on the rim and tip regions of the zeolite ZSM-5 crystals. On the basis of the confocal approach of the investigation, the aforementioned features are evaluated in three dimensions, while the degradation of the zeolite framework upon postsynthesis steam treatment could be visualized by occupation of the sinusoidal pores.
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spelling pubmed-44357832015-05-23 Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where? Sprung, Christoph Weckhuysen, Bert M. J Am Chem Soc [Image: see text] A detailed and systematic polarized confocal fluorescence microscopy investigation is presented on three batches of large coffin-shaped ZSM-5 crystals (i.e., parent, steamed at 500 °C, and steamed at 700 °C). In total, six laser lines of different wavelength in the visible region are employed on two crystal positions and three orientations with respect to the polarization plane of the excitation laser light. A fluorescent probe molecule is generated inside the zeolite pores, originating from the acid-catalyzed oligomerization of 4-fluorostyrene. A thorough analysis of the polarization plane of emitting fluorescent light reveals insight into the orientation of the fluorescent probe molecule restricted by the highly ordered zeolite channel framework, thereby visualizing pore accessibility and clearly distinguishing the occupation of straight and sinusoidal channels by the probe molecule. Spectral features are, furthermore, observed to tell apart molecules situated in one or the other pore. Special focus was given on the rim and tip regions of the zeolite ZSM-5 crystals. On the basis of the confocal approach of the investigation, the aforementioned features are evaluated in three dimensions, while the degradation of the zeolite framework upon postsynthesis steam treatment could be visualized by occupation of the sinusoidal pores. American Chemical Society 2015-01-15 2015-02-11 /pmc/articles/PMC4435783/ /pubmed/25590519 http://dx.doi.org/10.1021/ja511381f Text en Copyright © 2015 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Sprung, Christoph
Weckhuysen, Bert M.
Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?
title Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?
title_full Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?
title_fullStr Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?
title_full_unstemmed Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?
title_short Differences in the Location of Guest Molecules within Zeolite Pores As Revealed by Multilaser Excitation Confocal Fluorescence Microscopy: Which Molecule Is Where?
title_sort differences in the location of guest molecules within zeolite pores as revealed by multilaser excitation confocal fluorescence microscopy: which molecule is where?
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435783/
https://www.ncbi.nlm.nih.gov/pubmed/25590519
http://dx.doi.org/10.1021/ja511381f
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