Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons

Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to...

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Detalles Bibliográficos
Autores principales: Wetmore, Kelly M., Price, Morgan N., Waters, Robert J., Lamson, Jacob S., He, Jennifer, Hoover, Cindi A., Blow, Matthew J., Bristow, James, Butland, Gareth, Arkin, Adam P., Deutschbauer, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436071/
https://www.ncbi.nlm.nih.gov/pubmed/25968644
http://dx.doi.org/10.1128/mBio.00306-15

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436071/
https://www.ncbi.nlm.nih.gov/pubmed/25968644
http://dx.doi.org/10.1128/mBio.00306-15

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