Cargando…

Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs

Different toxic compounds can target the cholinergic nervous system. Acetylcholinesterase (AChE; EC 3.1.1.7) is one of the most crucial components of the cholinergic nervous system and thus many of the toxins interact with this enzyme. As to inhibitors, nerve agents used as chemical warfare, some in...

Descripción completa

Detalles Bibliográficos
Autores principales: Pohanka, Miroslav, Vlcek, Vitezslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Slovak Toxicology Society SETOX 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436211/
https://www.ncbi.nlm.nih.gov/pubmed/26109903
http://dx.doi.org/10.2478/intox-2014-0031
_version_ 1782372031245844480
author Pohanka, Miroslav
Vlcek, Vitezslav
author_facet Pohanka, Miroslav
Vlcek, Vitezslav
author_sort Pohanka, Miroslav
collection PubMed
description Different toxic compounds can target the cholinergic nervous system. Acetylcholinesterase (AChE; EC 3.1.1.7) is one of the most crucial components of the cholinergic nervous system and thus many of the toxins interact with this enzyme. As to inhibitors, nerve agents used as chemical warfare, some insecticides, and drugs influencing the cholinergic system are common examples of AChE inhibitors. Once inhibited by a neurotoxic compound, a serious cholinergic crisis can occur. On the other hand, sensitivity of AChE to the inhibition can be used for analytical purposes. In this study, a simple disposable biosensor with AChE as a recognition element was devised. AChE was immobilized onto a cellulose matrix and indoxylacetate was used as a chromogenic substrate. The enzyme reaction was assessed by the naked eye using arbitrary units and pyridostigmine, tacrine, paraoxon, carbofuran, soman and VX were assayed as selected inhibitors. A good stability of the biosensors was found, with no aging over a quarter of a year and minimal sensitivity to the interference of organic solvents. The limit of detection ranged from 10 to 100 nmol/L for the compounds tested with a sample volume of 40 µL.
format Online
Article
Text
id pubmed-4436211
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Slovak Toxicology Society SETOX
record_format MEDLINE/PubMed
spelling pubmed-44362112015-06-24 Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs Pohanka, Miroslav Vlcek, Vitezslav Interdiscip Toxicol Original Article Different toxic compounds can target the cholinergic nervous system. Acetylcholinesterase (AChE; EC 3.1.1.7) is one of the most crucial components of the cholinergic nervous system and thus many of the toxins interact with this enzyme. As to inhibitors, nerve agents used as chemical warfare, some insecticides, and drugs influencing the cholinergic system are common examples of AChE inhibitors. Once inhibited by a neurotoxic compound, a serious cholinergic crisis can occur. On the other hand, sensitivity of AChE to the inhibition can be used for analytical purposes. In this study, a simple disposable biosensor with AChE as a recognition element was devised. AChE was immobilized onto a cellulose matrix and indoxylacetate was used as a chromogenic substrate. The enzyme reaction was assessed by the naked eye using arbitrary units and pyridostigmine, tacrine, paraoxon, carbofuran, soman and VX were assayed as selected inhibitors. A good stability of the biosensors was found, with no aging over a quarter of a year and minimal sensitivity to the interference of organic solvents. The limit of detection ranged from 10 to 100 nmol/L for the compounds tested with a sample volume of 40 µL. Slovak Toxicology Society SETOX 2014-12 2015-03-04 /pmc/articles/PMC4436211/ /pubmed/26109903 http://dx.doi.org/10.2478/intox-2014-0031 Text en Copyright © 2014 SETOX & Institute of Experimental Pharmacology and Toxicology, SASc. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pohanka, Miroslav
Vlcek, Vitezslav
Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
title Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
title_full Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
title_fullStr Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
title_full_unstemmed Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
title_short Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
title_sort preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436211/
https://www.ncbi.nlm.nih.gov/pubmed/26109903
http://dx.doi.org/10.2478/intox-2014-0031
work_keys_str_mv AT pohankamiroslav preparationandperformanceofacolorimetricbiosensorusingacetylcholinesteraseandindoxylacetateforassayofnerveagentsanddrugs
AT vlcekvitezslav preparationandperformanceofacolorimetricbiosensorusingacetylcholinesteraseandindoxylacetateforassayofnerveagentsanddrugs