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Microtubule-dependent transport and dynamics of vimentin intermediate filaments
We studied two aspects of vimentin intermediate filament dynamics—transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertib...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436779/ https://www.ncbi.nlm.nih.gov/pubmed/25717187 http://dx.doi.org/10.1091/mbc.E14-09-1398 |
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author | Hookway, Caroline Ding, Liya Davidson, Michael W. Rappoport, Joshua Z. Danuser, Gaudenz Gelfand, Vladimir I. |
author_facet | Hookway, Caroline Ding, Liya Davidson, Michael W. Rappoport, Joshua Z. Danuser, Gaudenz Gelfand, Vladimir I. |
author_sort | Hookway, Caroline |
collection | PubMed |
description | We studied two aspects of vimentin intermediate filament dynamics—transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments were rapidly transported along linear tracks in both anterograde and retrograde directions. Filament transport was microtubule dependent but independent of microtubule polymerization and/or an interaction with the plus end–binding protein APC. We also studied subunit exchange in filaments by long-term imaging after photoconversion. We found that converted vimentin remained in small clusters along the length of filaments rather than redistributing uniformly throughout the network, even in cells that divided after photoconversion. These data show that vimentin filaments do not depolymerize into individual subunits; they recompose by severing and reannealing. Together these results show that vimentin filaments are very dynamic and that their transport is required for network maintenance. |
format | Online Article Text |
id | pubmed-4436779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-44367792015-07-16 Microtubule-dependent transport and dynamics of vimentin intermediate filaments Hookway, Caroline Ding, Liya Davidson, Michael W. Rappoport, Joshua Z. Danuser, Gaudenz Gelfand, Vladimir I. Mol Biol Cell Articles We studied two aspects of vimentin intermediate filament dynamics—transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments were rapidly transported along linear tracks in both anterograde and retrograde directions. Filament transport was microtubule dependent but independent of microtubule polymerization and/or an interaction with the plus end–binding protein APC. We also studied subunit exchange in filaments by long-term imaging after photoconversion. We found that converted vimentin remained in small clusters along the length of filaments rather than redistributing uniformly throughout the network, even in cells that divided after photoconversion. These data show that vimentin filaments do not depolymerize into individual subunits; they recompose by severing and reannealing. Together these results show that vimentin filaments are very dynamic and that their transport is required for network maintenance. The American Society for Cell Biology 2015-05-01 /pmc/articles/PMC4436779/ /pubmed/25717187 http://dx.doi.org/10.1091/mbc.E14-09-1398 Text en © 2015 Hookway et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Hookway, Caroline Ding, Liya Davidson, Michael W. Rappoport, Joshua Z. Danuser, Gaudenz Gelfand, Vladimir I. Microtubule-dependent transport and dynamics of vimentin intermediate filaments |
title | Microtubule-dependent transport and dynamics of vimentin intermediate filaments |
title_full | Microtubule-dependent transport and dynamics of vimentin intermediate filaments |
title_fullStr | Microtubule-dependent transport and dynamics of vimentin intermediate filaments |
title_full_unstemmed | Microtubule-dependent transport and dynamics of vimentin intermediate filaments |
title_short | Microtubule-dependent transport and dynamics of vimentin intermediate filaments |
title_sort | microtubule-dependent transport and dynamics of vimentin intermediate filaments |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436779/ https://www.ncbi.nlm.nih.gov/pubmed/25717187 http://dx.doi.org/10.1091/mbc.E14-09-1398 |
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