Cargando…

Microtubule-dependent transport and dynamics of vimentin intermediate filaments

We studied two aspects of vimentin intermediate filament dynamics—transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertib...

Descripción completa

Detalles Bibliográficos
Autores principales: Hookway, Caroline, Ding, Liya, Davidson, Michael W., Rappoport, Joshua Z., Danuser, Gaudenz, Gelfand, Vladimir I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436779/
https://www.ncbi.nlm.nih.gov/pubmed/25717187
http://dx.doi.org/10.1091/mbc.E14-09-1398
_version_ 1782372128802209792
author Hookway, Caroline
Ding, Liya
Davidson, Michael W.
Rappoport, Joshua Z.
Danuser, Gaudenz
Gelfand, Vladimir I.
author_facet Hookway, Caroline
Ding, Liya
Davidson, Michael W.
Rappoport, Joshua Z.
Danuser, Gaudenz
Gelfand, Vladimir I.
author_sort Hookway, Caroline
collection PubMed
description We studied two aspects of vimentin intermediate filament dynamics—transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments were rapidly transported along linear tracks in both anterograde and retrograde directions. Filament transport was microtubule dependent but independent of microtubule polymerization and/or an interaction with the plus end–binding protein APC. We also studied subunit exchange in filaments by long-term imaging after photoconversion. We found that converted vimentin remained in small clusters along the length of filaments rather than redistributing uniformly throughout the network, even in cells that divided after photoconversion. These data show that vimentin filaments do not depolymerize into individual subunits; they recompose by severing and reannealing. Together these results show that vimentin filaments are very dynamic and that their transport is required for network maintenance.
format Online
Article
Text
id pubmed-4436779
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-44367792015-07-16 Microtubule-dependent transport and dynamics of vimentin intermediate filaments Hookway, Caroline Ding, Liya Davidson, Michael W. Rappoport, Joshua Z. Danuser, Gaudenz Gelfand, Vladimir I. Mol Biol Cell Articles We studied two aspects of vimentin intermediate filament dynamics—transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments were rapidly transported along linear tracks in both anterograde and retrograde directions. Filament transport was microtubule dependent but independent of microtubule polymerization and/or an interaction with the plus end–binding protein APC. We also studied subunit exchange in filaments by long-term imaging after photoconversion. We found that converted vimentin remained in small clusters along the length of filaments rather than redistributing uniformly throughout the network, even in cells that divided after photoconversion. These data show that vimentin filaments do not depolymerize into individual subunits; they recompose by severing and reannealing. Together these results show that vimentin filaments are very dynamic and that their transport is required for network maintenance. The American Society for Cell Biology 2015-05-01 /pmc/articles/PMC4436779/ /pubmed/25717187 http://dx.doi.org/10.1091/mbc.E14-09-1398 Text en © 2015 Hookway et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Hookway, Caroline
Ding, Liya
Davidson, Michael W.
Rappoport, Joshua Z.
Danuser, Gaudenz
Gelfand, Vladimir I.
Microtubule-dependent transport and dynamics of vimentin intermediate filaments
title Microtubule-dependent transport and dynamics of vimentin intermediate filaments
title_full Microtubule-dependent transport and dynamics of vimentin intermediate filaments
title_fullStr Microtubule-dependent transport and dynamics of vimentin intermediate filaments
title_full_unstemmed Microtubule-dependent transport and dynamics of vimentin intermediate filaments
title_short Microtubule-dependent transport and dynamics of vimentin intermediate filaments
title_sort microtubule-dependent transport and dynamics of vimentin intermediate filaments
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436779/
https://www.ncbi.nlm.nih.gov/pubmed/25717187
http://dx.doi.org/10.1091/mbc.E14-09-1398
work_keys_str_mv AT hookwaycaroline microtubuledependenttransportanddynamicsofvimentinintermediatefilaments
AT dingliya microtubuledependenttransportanddynamicsofvimentinintermediatefilaments
AT davidsonmichaelw microtubuledependenttransportanddynamicsofvimentinintermediatefilaments
AT rappoportjoshuaz microtubuledependenttransportanddynamicsofvimentinintermediatefilaments
AT danusergaudenz microtubuledependenttransportanddynamicsofvimentinintermediatefilaments
AT gelfandvladimiri microtubuledependenttransportanddynamicsofvimentinintermediatefilaments