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Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture

Fascin, a highly conserved actin-bundling protein, localizes and functions at new cellular sites in both Drosophila and multiple mammalian cell types. During Drosophila follicle development, in addition to being cytoplasmic, Fascin is in the nuclei of the germline-derived nurse cells during stages 1...

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Autores principales: Groen, Christopher M., Jayo, Asier, Parsons, Maddy, Tootle, Tina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436834/
https://www.ncbi.nlm.nih.gov/pubmed/25808493
http://dx.doi.org/10.1091/mbc.E14-09-1384
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author Groen, Christopher M.
Jayo, Asier
Parsons, Maddy
Tootle, Tina L.
author_facet Groen, Christopher M.
Jayo, Asier
Parsons, Maddy
Tootle, Tina L.
author_sort Groen, Christopher M.
collection PubMed
description Fascin, a highly conserved actin-bundling protein, localizes and functions at new cellular sites in both Drosophila and multiple mammalian cell types. During Drosophila follicle development, in addition to being cytoplasmic, Fascin is in the nuclei of the germline-derived nurse cells during stages 10B–12 (S10B–12) and at the nuclear periphery during stage 13 (S13). This localization is specific to Fascin, as other actin-binding proteins, Villin and Profilin, do not exhibit the same subcellular distribution. In addition, localization of fascin1 to the nucleus and nuclear periphery is observed in multiple mammalian cell types. Thus the regulation and function of Fascin at these new cellular locations is likely to be highly conserved. In Drosophila, loss of prostaglandin signaling causes a global reduction in nuclear Fascin and a failure to relocalize to the nuclear periphery. Alterations in nuclear Fascin levels result in defects in nucleolar morphology in both Drosophila follicles and cultured mammalian cells, suggesting that nuclear Fascin plays an important role in nucleolar architecture. Given the numerous roles of Fascin in development and disease, including cancer, our novel finding that Fascin has functions within the nucleus sheds new light on the potential roles of Fascin in these contexts.
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spelling pubmed-44368342015-07-30 Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture Groen, Christopher M. Jayo, Asier Parsons, Maddy Tootle, Tina L. Mol Biol Cell Articles Fascin, a highly conserved actin-bundling protein, localizes and functions at new cellular sites in both Drosophila and multiple mammalian cell types. During Drosophila follicle development, in addition to being cytoplasmic, Fascin is in the nuclei of the germline-derived nurse cells during stages 10B–12 (S10B–12) and at the nuclear periphery during stage 13 (S13). This localization is specific to Fascin, as other actin-binding proteins, Villin and Profilin, do not exhibit the same subcellular distribution. In addition, localization of fascin1 to the nucleus and nuclear periphery is observed in multiple mammalian cell types. Thus the regulation and function of Fascin at these new cellular locations is likely to be highly conserved. In Drosophila, loss of prostaglandin signaling causes a global reduction in nuclear Fascin and a failure to relocalize to the nuclear periphery. Alterations in nuclear Fascin levels result in defects in nucleolar morphology in both Drosophila follicles and cultured mammalian cells, suggesting that nuclear Fascin plays an important role in nucleolar architecture. Given the numerous roles of Fascin in development and disease, including cancer, our novel finding that Fascin has functions within the nucleus sheds new light on the potential roles of Fascin in these contexts. The American Society for Cell Biology 2015-05-15 /pmc/articles/PMC4436834/ /pubmed/25808493 http://dx.doi.org/10.1091/mbc.E14-09-1384 Text en © 2015 Groen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Groen, Christopher M.
Jayo, Asier
Parsons, Maddy
Tootle, Tina L.
Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
title Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
title_full Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
title_fullStr Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
title_full_unstemmed Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
title_short Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
title_sort prostaglandins regulate nuclear localization of fascin and its function in nucleolar architecture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436834/
https://www.ncbi.nlm.nih.gov/pubmed/25808493
http://dx.doi.org/10.1091/mbc.E14-09-1384
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