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SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors
SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci–infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP–transfected HeLa cells indicate that SINC targets the inner n...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436835/ https://www.ncbi.nlm.nih.gov/pubmed/25788290 http://dx.doi.org/10.1091/mbc.E14-11-1530 |
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author | Mojica, Sergio A. Hovis, Kelley M. Frieman, Matthew B. Tran, Bao Hsia, Ru-ching Ravel, Jacques Jenkins-Houk, Clifton Wilson, Katherine L. Bavoil, Patrik M. |
author_facet | Mojica, Sergio A. Hovis, Kelley M. Frieman, Matthew B. Tran, Bao Hsia, Ru-ching Ravel, Jacques Jenkins-Houk, Clifton Wilson, Katherine L. Bavoil, Patrik M. |
author_sort | Mojica, Sergio A. |
collection | PubMed |
description | SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci–infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP–transfected HeLa cells indicate that SINC targets the inner nuclear membrane. SINC localization at the nuclear envelope was blocked by importazole, confirming SINC import into the nucleus. Candidate partners were identified by proximity to biotin ligase-fused SINC in HEK293 cells and mass spectrometry (BioID). This strategy identified 22 candidates with high confidence, including the nucleoporin ELYS, lamin B1, and four proteins (emerin, MAN1, LAP1, and LBR) of the inner nuclear membrane, suggesting that SINC interacts with host proteins that control nuclear structure, signaling, chromatin organization, and gene silencing. GFP-SINC association with the native LEM-domain protein emerin, a conserved component of nuclear “lamina” structure, or with a complex containing emerin was confirmed by GFP pull down. Our findings identify SINC as a novel bacterial protein that targets the nuclear envelope with the capability of globally altering nuclear envelope functions in the infected host cell and neighboring uninfected cells. These properties may contribute to the aggressive virulence of C. psittaci. |
format | Online Article Text |
id | pubmed-4436835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-44368352015-07-30 SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors Mojica, Sergio A. Hovis, Kelley M. Frieman, Matthew B. Tran, Bao Hsia, Ru-ching Ravel, Jacques Jenkins-Houk, Clifton Wilson, Katherine L. Bavoil, Patrik M. Mol Biol Cell Articles SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci–infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP–transfected HeLa cells indicate that SINC targets the inner nuclear membrane. SINC localization at the nuclear envelope was blocked by importazole, confirming SINC import into the nucleus. Candidate partners were identified by proximity to biotin ligase-fused SINC in HEK293 cells and mass spectrometry (BioID). This strategy identified 22 candidates with high confidence, including the nucleoporin ELYS, lamin B1, and four proteins (emerin, MAN1, LAP1, and LBR) of the inner nuclear membrane, suggesting that SINC interacts with host proteins that control nuclear structure, signaling, chromatin organization, and gene silencing. GFP-SINC association with the native LEM-domain protein emerin, a conserved component of nuclear “lamina” structure, or with a complex containing emerin was confirmed by GFP pull down. Our findings identify SINC as a novel bacterial protein that targets the nuclear envelope with the capability of globally altering nuclear envelope functions in the infected host cell and neighboring uninfected cells. These properties may contribute to the aggressive virulence of C. psittaci. The American Society for Cell Biology 2015-05-15 /pmc/articles/PMC4436835/ /pubmed/25788290 http://dx.doi.org/10.1091/mbc.E14-11-1530 Text en © 2015 Mojica et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Mojica, Sergio A. Hovis, Kelley M. Frieman, Matthew B. Tran, Bao Hsia, Ru-ching Ravel, Jacques Jenkins-Houk, Clifton Wilson, Katherine L. Bavoil, Patrik M. SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
title | SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
title_full | SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
title_fullStr | SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
title_full_unstemmed | SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
title_short | SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
title_sort | sinc, a type iii secreted protein of chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436835/ https://www.ncbi.nlm.nih.gov/pubmed/25788290 http://dx.doi.org/10.1091/mbc.E14-11-1530 |
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