Cargando…
Seasonal variation of bacterial endophytes in urban trees
Bacterial endophytes, non-pathogenic bacteria residing within plants, contribute to the growth and development of plants and their ability to adapt to adverse conditions. In order to fully exploit the capabilities of these bacteria, it is necessary to understand the extent to which endophytic commun...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437045/ https://www.ncbi.nlm.nih.gov/pubmed/26042095 http://dx.doi.org/10.3389/fmicb.2015.00427 |
_version_ | 1782372169231106048 |
---|---|
author | Shen, Shu Yi Fulthorpe, Roberta |
author_facet | Shen, Shu Yi Fulthorpe, Roberta |
author_sort | Shen, Shu Yi |
collection | PubMed |
description | Bacterial endophytes, non-pathogenic bacteria residing within plants, contribute to the growth and development of plants and their ability to adapt to adverse conditions. In order to fully exploit the capabilities of these bacteria, it is necessary to understand the extent to which endophytic communities vary between species and over time. The endophytes of Acer negundo, Ulmus pumila, and Ulmus parvifolia were sampled over three seasons and analyzed using culture dependent and independent methods (culture on two media, terminal restriction fragment length polymorphism, and tagged pyrosequencing of 16S ribosomal amplicons). The majority of culturable endophytes isolated were Actinobacteria, and all the samples harbored Bacillus, Curtobacterium, Frigoribacterium, Methylobacterium, Paenibacilllus, and Sphingomonas species. Regardless of culture medium used, only the culturable communities obtained in the winter for A. negundo could be distinguished from those of Ulmus spp. In contrast, the nonculturable communities were dominated by Proteobacteria and Actinobacteria, particularly Erwinia, Ralstonia, and Sanguibacter spp. The presence and abundance of various bacterial classes and phyla changed with the changing seasons. Multivariate analysis on the culture independent data revealed significant community differences between the endophytic communities of A. negundo and Ulmus spp., but overall season was the main determinant of endophytic community structure. This study suggests studies on endophytic populations of urban trees should expect to find significant seasonal and species-specific community differences and sampling should proceed accordingly. |
format | Online Article Text |
id | pubmed-4437045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44370452015-06-03 Seasonal variation of bacterial endophytes in urban trees Shen, Shu Yi Fulthorpe, Roberta Front Microbiol Plant Science Bacterial endophytes, non-pathogenic bacteria residing within plants, contribute to the growth and development of plants and their ability to adapt to adverse conditions. In order to fully exploit the capabilities of these bacteria, it is necessary to understand the extent to which endophytic communities vary between species and over time. The endophytes of Acer negundo, Ulmus pumila, and Ulmus parvifolia were sampled over three seasons and analyzed using culture dependent and independent methods (culture on two media, terminal restriction fragment length polymorphism, and tagged pyrosequencing of 16S ribosomal amplicons). The majority of culturable endophytes isolated were Actinobacteria, and all the samples harbored Bacillus, Curtobacterium, Frigoribacterium, Methylobacterium, Paenibacilllus, and Sphingomonas species. Regardless of culture medium used, only the culturable communities obtained in the winter for A. negundo could be distinguished from those of Ulmus spp. In contrast, the nonculturable communities were dominated by Proteobacteria and Actinobacteria, particularly Erwinia, Ralstonia, and Sanguibacter spp. The presence and abundance of various bacterial classes and phyla changed with the changing seasons. Multivariate analysis on the culture independent data revealed significant community differences between the endophytic communities of A. negundo and Ulmus spp., but overall season was the main determinant of endophytic community structure. This study suggests studies on endophytic populations of urban trees should expect to find significant seasonal and species-specific community differences and sampling should proceed accordingly. Frontiers Media S.A. 2015-05-19 /pmc/articles/PMC4437045/ /pubmed/26042095 http://dx.doi.org/10.3389/fmicb.2015.00427 Text en Copyright © 2015 Shen and Fulthorpe. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Shen, Shu Yi Fulthorpe, Roberta Seasonal variation of bacterial endophytes in urban trees |
title | Seasonal variation of bacterial endophytes in urban trees |
title_full | Seasonal variation of bacterial endophytes in urban trees |
title_fullStr | Seasonal variation of bacterial endophytes in urban trees |
title_full_unstemmed | Seasonal variation of bacterial endophytes in urban trees |
title_short | Seasonal variation of bacterial endophytes in urban trees |
title_sort | seasonal variation of bacterial endophytes in urban trees |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437045/ https://www.ncbi.nlm.nih.gov/pubmed/26042095 http://dx.doi.org/10.3389/fmicb.2015.00427 |
work_keys_str_mv | AT shenshuyi seasonalvariationofbacterialendophytesinurbantrees AT fulthorperoberta seasonalvariationofbacterialendophytesinurbantrees |