Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference

The first reported Type IV restriction endonuclease (REase) GmrSD consists of GmrS and GmrD subunits. In most bacteria, however, the gmrS and gmrD genes are fused together to encode a single-chain protein. The fused coding sequence for ECSTEC94C_1402 from E. coli strain STEC_94C was expressed in T7...

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Detalles Bibliográficos
Autores principales: He, Xinyi, Hull, Victoria, Thomas, Julie A., Fu, Xiaoqing, Gidwani, Sonal, Gupta, Yogesh K., Black, Lindsay W., Xu, Shuang-yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437046/
https://www.ncbi.nlm.nih.gov/pubmed/25988532
http://dx.doi.org/10.1038/srep09747

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