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Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering

Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical mod...

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Autores principales: Lienemann, Philipp S., Metzger, Stéphanie, Kiveliö, Anna-Sofia, Blanc, Alain, Papageorgiou, Panagiota, Astolfo, Alberto, Pinzer, Bernd R., Cinelli, Paolo, Weber, Franz E., Schibli, Roger, Béhé, Martin, Ehrbar, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437296/
https://www.ncbi.nlm.nih.gov/pubmed/25989250
http://dx.doi.org/10.1038/srep10238
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author Lienemann, Philipp S.
Metzger, Stéphanie
Kiveliö, Anna-Sofia
Blanc, Alain
Papageorgiou, Panagiota
Astolfo, Alberto
Pinzer, Bernd R.
Cinelli, Paolo
Weber, Franz E.
Schibli, Roger
Béhé, Martin
Ehrbar, Martin
author_facet Lienemann, Philipp S.
Metzger, Stéphanie
Kiveliö, Anna-Sofia
Blanc, Alain
Papageorgiou, Panagiota
Astolfo, Alberto
Pinzer, Bernd R.
Cinelli, Paolo
Weber, Franz E.
Schibli, Roger
Béhé, Martin
Ehrbar, Martin
author_sort Lienemann, Philipp S.
collection PubMed
description Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.
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spelling pubmed-44372962015-06-01 Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering Lienemann, Philipp S. Metzger, Stéphanie Kiveliö, Anna-Sofia Blanc, Alain Papageorgiou, Panagiota Astolfo, Alberto Pinzer, Bernd R. Cinelli, Paolo Weber, Franz E. Schibli, Roger Béhé, Martin Ehrbar, Martin Sci Rep Article Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials. Nature Publishing Group 2015-05-19 /pmc/articles/PMC4437296/ /pubmed/25989250 http://dx.doi.org/10.1038/srep10238 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lienemann, Philipp S.
Metzger, Stéphanie
Kiveliö, Anna-Sofia
Blanc, Alain
Papageorgiou, Panagiota
Astolfo, Alberto
Pinzer, Bernd R.
Cinelli, Paolo
Weber, Franz E.
Schibli, Roger
Béhé, Martin
Ehrbar, Martin
Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering
title Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering
title_full Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering
title_fullStr Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering
title_full_unstemmed Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering
title_short Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering
title_sort longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole spect and micro-ct for tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437296/
https://www.ncbi.nlm.nih.gov/pubmed/25989250
http://dx.doi.org/10.1038/srep10238
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