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Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin

The expression of N-glycolylneuraminic acid forming the structure of gangliosides and/or other glycoconjugates (Hanganutziu-Deicher antigen) in human has been considered as a tumor-associated antigen. Specifically, some reports of 14F7 Mab (a highly specific Mab raised against N-glycolyl GM3 ganglio...

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Autores principales: Blanco, Rancés, Quintana, Yisel, Blanco, Damián, Cedeño, Mercedes, Rengifo, Charles E., Frómeta, Milagros, Ríos, Martha, Rengifo, Enrique, Carr, Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437369/
https://www.ncbi.nlm.nih.gov/pubmed/26317019
http://dx.doi.org/10.1155/2013/602417
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author Blanco, Rancés
Quintana, Yisel
Blanco, Damián
Cedeño, Mercedes
Rengifo, Charles E.
Frómeta, Milagros
Ríos, Martha
Rengifo, Enrique
Carr, Adriana
author_facet Blanco, Rancés
Quintana, Yisel
Blanco, Damián
Cedeño, Mercedes
Rengifo, Charles E.
Frómeta, Milagros
Ríos, Martha
Rengifo, Enrique
Carr, Adriana
author_sort Blanco, Rancés
collection PubMed
description The expression of N-glycolylneuraminic acid forming the structure of gangliosides and/or other glycoconjugates (Hanganutziu-Deicher antigen) in human has been considered as a tumor-associated antigen. Specifically, some reports of 14F7 Mab (a highly specific Mab raised against N-glycolyl GM3 ganglioside) reactivity in human tumors have been recently published. Nevertheless, tumors of epithelial origin have been mostly evaluated. The goal of the present paper was to evaluate the immunohistochemical recognition of 14F7 Mab in different human tumors of neuroectodermal, mesodermal, and epithelial origins using an immunoperoxidase staining method. Samples of fetal, normal, and reactive astrocytosis of the brain were also included in the study. In general, nontumoral tissues, as well as, low-grade brain tumors showed no or a limited immunoreaction with 14F7 Mab. Nevertheless, high-grade astrocytomas (III-IV) and neuroblastomas, as well as, sarcomas and thyroid carcinomas were mostly reactive with 14F7. No reaction was evidenced in medulloblastomas and ependymoblastomas. Our data suggest that the expression of N-glycolyl GM3 ganglioside could be related to the aggressive behavior of malignant cells, without depending on the tumor origin. Our data could also support the possible use of N-glycolyl GM3 as a target for both active and passive immunotherapies of malignancies expressing this molecule.
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spelling pubmed-44373692015-08-27 Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin Blanco, Rancés Quintana, Yisel Blanco, Damián Cedeño, Mercedes Rengifo, Charles E. Frómeta, Milagros Ríos, Martha Rengifo, Enrique Carr, Adriana J Biomark Research Article The expression of N-glycolylneuraminic acid forming the structure of gangliosides and/or other glycoconjugates (Hanganutziu-Deicher antigen) in human has been considered as a tumor-associated antigen. Specifically, some reports of 14F7 Mab (a highly specific Mab raised against N-glycolyl GM3 ganglioside) reactivity in human tumors have been recently published. Nevertheless, tumors of epithelial origin have been mostly evaluated. The goal of the present paper was to evaluate the immunohistochemical recognition of 14F7 Mab in different human tumors of neuroectodermal, mesodermal, and epithelial origins using an immunoperoxidase staining method. Samples of fetal, normal, and reactive astrocytosis of the brain were also included in the study. In general, nontumoral tissues, as well as, low-grade brain tumors showed no or a limited immunoreaction with 14F7 Mab. Nevertheless, high-grade astrocytomas (III-IV) and neuroblastomas, as well as, sarcomas and thyroid carcinomas were mostly reactive with 14F7. No reaction was evidenced in medulloblastomas and ependymoblastomas. Our data suggest that the expression of N-glycolyl GM3 ganglioside could be related to the aggressive behavior of malignant cells, without depending on the tumor origin. Our data could also support the possible use of N-glycolyl GM3 as a target for both active and passive immunotherapies of malignancies expressing this molecule. Hindawi Publishing Corporation 2013 2013-01-09 /pmc/articles/PMC4437369/ /pubmed/26317019 http://dx.doi.org/10.1155/2013/602417 Text en Copyright © 2013 Rancés Blanco et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Blanco, Rancés
Quintana, Yisel
Blanco, Damián
Cedeño, Mercedes
Rengifo, Charles E.
Frómeta, Milagros
Ríos, Martha
Rengifo, Enrique
Carr, Adriana
Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin
title Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin
title_full Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin
title_fullStr Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin
title_full_unstemmed Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin
title_short Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin
title_sort tissue reactivity of the 14f7 mab raised against n-glycolyl gm3 ganglioside in tumors of neuroectodermal, mesodermal, and epithelial origin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437369/
https://www.ncbi.nlm.nih.gov/pubmed/26317019
http://dx.doi.org/10.1155/2013/602417
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