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DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis

BACKGROUND: Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are systemic autoimmune connective tissue diseases that share overlapping clinico-pathological features. It is highly probable that there is an overlap in epigenetic landscapes of both diseases. This study aimed to identify...

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Autores principales: Matatiele, Puleng, Tikly, Mohamed, Tarr, Gareth, Gulumian, Mary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437745/
https://www.ncbi.nlm.nih.gov/pubmed/25986394
http://dx.doi.org/10.1186/s12929-015-0142-2
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author Matatiele, Puleng
Tikly, Mohamed
Tarr, Gareth
Gulumian, Mary
author_facet Matatiele, Puleng
Tikly, Mohamed
Tarr, Gareth
Gulumian, Mary
author_sort Matatiele, Puleng
collection PubMed
description BACKGROUND: Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are systemic autoimmune connective tissue diseases that share overlapping clinico-pathological features. It is highly probable that there is an overlap in epigenetic landscapes of both diseases. This study aimed to identify similarities in DNA methylation changes in genes involved in SLE and SSc. Global DNA methylation and twelve genes selected on the basis of their involvement in inflammation, autoimmunity and/or fibrosis were analyzed using PCR arrays in three groups, each of 30 Black South Africans with SLE and SSc, plus 40 healthy control subjects. RESULTS: Global methylation in both diseases was significantly lower (<25 %) than in healthy subjects (>30 %, p = 0.0000001). In comparison to healthy controls, a similar gene-specific methylation pattern was observed in both SLE and SSc. Three genes, namely; PRF1, ITGAL and FOXP3 were consistently hypermethylated while CDKN2A and CD70 were hypomethylated in both diseases. The other genes (SOCS1, CTGF, THY1, CXCR4, MT1-G, FLI1, and DNMT1) were generally hypomethylated in SLE whereas they were neither hyper- nor hypo-methylated in SSc. CONCLUSIONS: SSc and SLE patients have a higher global hypomethylation than healthy subjects with specific genes being hypomethylated and others hypermethylated. The majority of genes studied were hypomethylated in SLE compared to SSc. In addition to the commonly known hypomethylated genes in SLE and SSc, there are other hypomethylated genes (such as MT-1G and THY-1) that have not previously been investigated in SLE and SSc though are known to be hypermethylated in cancer.
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spelling pubmed-44377452015-05-20 DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis Matatiele, Puleng Tikly, Mohamed Tarr, Gareth Gulumian, Mary J Biomed Sci Research BACKGROUND: Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are systemic autoimmune connective tissue diseases that share overlapping clinico-pathological features. It is highly probable that there is an overlap in epigenetic landscapes of both diseases. This study aimed to identify similarities in DNA methylation changes in genes involved in SLE and SSc. Global DNA methylation and twelve genes selected on the basis of their involvement in inflammation, autoimmunity and/or fibrosis were analyzed using PCR arrays in three groups, each of 30 Black South Africans with SLE and SSc, plus 40 healthy control subjects. RESULTS: Global methylation in both diseases was significantly lower (<25 %) than in healthy subjects (>30 %, p = 0.0000001). In comparison to healthy controls, a similar gene-specific methylation pattern was observed in both SLE and SSc. Three genes, namely; PRF1, ITGAL and FOXP3 were consistently hypermethylated while CDKN2A and CD70 were hypomethylated in both diseases. The other genes (SOCS1, CTGF, THY1, CXCR4, MT1-G, FLI1, and DNMT1) were generally hypomethylated in SLE whereas they were neither hyper- nor hypo-methylated in SSc. CONCLUSIONS: SSc and SLE patients have a higher global hypomethylation than healthy subjects with specific genes being hypomethylated and others hypermethylated. The majority of genes studied were hypomethylated in SLE compared to SSc. In addition to the commonly known hypomethylated genes in SLE and SSc, there are other hypomethylated genes (such as MT-1G and THY-1) that have not previously been investigated in SLE and SSc though are known to be hypermethylated in cancer. BioMed Central 2015-05-20 /pmc/articles/PMC4437745/ /pubmed/25986394 http://dx.doi.org/10.1186/s12929-015-0142-2 Text en © Matatiele et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Matatiele, Puleng
Tikly, Mohamed
Tarr, Gareth
Gulumian, Mary
DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis
title DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis
title_full DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis
title_fullStr DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis
title_full_unstemmed DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis
title_short DNA methylation similarities in genes of black South Africans with systemic lupus erythematosus and systemic sclerosis
title_sort dna methylation similarities in genes of black south africans with systemic lupus erythematosus and systemic sclerosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437745/
https://www.ncbi.nlm.nih.gov/pubmed/25986394
http://dx.doi.org/10.1186/s12929-015-0142-2
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