Cargando…
S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis
Non-alcoholic fatty liver (NAFL) has the potential to progress to non-alcoholic steatohepatitis (NASH) or to promote type 2 diabetes mellitus (T2DM). However, NASH and T2DM do not always develop coordinately. Additionally, there are no definite noninvasive methods for NASH diagnosis currently. We es...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437778/ https://www.ncbi.nlm.nih.gov/pubmed/25993652 http://dx.doi.org/10.1371/journal.pone.0127352 |
_version_ | 1782372254824267776 |
---|---|
author | Liu, Xiaolin Wang, Yongfeng Ming, Yanan Song, Yanyan Zhang, Jingyi Chen, Xiaoyu Zeng, Minde Mao, Yimin |
author_facet | Liu, Xiaolin Wang, Yongfeng Ming, Yanan Song, Yanyan Zhang, Jingyi Chen, Xiaoyu Zeng, Minde Mao, Yimin |
author_sort | Liu, Xiaolin |
collection | PubMed |
description | Non-alcoholic fatty liver (NAFL) has the potential to progress to non-alcoholic steatohepatitis (NASH) or to promote type 2 diabetes mellitus (T2DM). However, NASH and T2DM do not always develop coordinately. Additionally, there are no definite noninvasive methods for NASH diagnosis currently. We established rat models of NAFL, NASH, and NAFL + T2DM to recapitulate different phenotypes associated with non-alcoholic fatty liver disease (NAFLD) and its progression. Histologic features of rat livers were scored according to criteria established by the Nonalcoholic Steatohepatitis Clinical Research Network. Microarray was performed to assess gene expression changes in rat livers. We find that gene expression of s100a9 was higher in NAFL group compared with control, and was increased in NASH groups and decreased in NAFL + T2DM group compared with NAFL. In contrast, srebf1, tbx21, and gimap4 only showed limited discriminating abilities in different groups. There is a significant positive correlation between serum levels of S100A9 and NAFLD Activity Score (NAS), the severity of hepatic steatosis, and lobular inflammation (r = 0.80, 0.64 and 0.86, P < 0.001). These findings suggest that S100A9 may be extremely useful in the diagnosis of NASH (AUROC: 0.947, CI: 0.845-1.049). Additionally, serum S100A9 levels displayed a strong correlation with ALT, AST and TBil (r = 0.81, 0.89 and 0.91, P < 0.001) but a weak correlation with FBG, HOMA-IR, TG, and TC (r = -0.41, -0.40, 0.47 and 0.49, P < 0.05). Conclusions: The results we provide here suggest that S100A9 may be useful as a biomarker for the hepatic and metabolic progression of NAFLD and the non-invasive diagnosis of NASH. |
format | Online Article Text |
id | pubmed-4437778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44377782015-05-29 S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis Liu, Xiaolin Wang, Yongfeng Ming, Yanan Song, Yanyan Zhang, Jingyi Chen, Xiaoyu Zeng, Minde Mao, Yimin PLoS One Research Article Non-alcoholic fatty liver (NAFL) has the potential to progress to non-alcoholic steatohepatitis (NASH) or to promote type 2 diabetes mellitus (T2DM). However, NASH and T2DM do not always develop coordinately. Additionally, there are no definite noninvasive methods for NASH diagnosis currently. We established rat models of NAFL, NASH, and NAFL + T2DM to recapitulate different phenotypes associated with non-alcoholic fatty liver disease (NAFLD) and its progression. Histologic features of rat livers were scored according to criteria established by the Nonalcoholic Steatohepatitis Clinical Research Network. Microarray was performed to assess gene expression changes in rat livers. We find that gene expression of s100a9 was higher in NAFL group compared with control, and was increased in NASH groups and decreased in NAFL + T2DM group compared with NAFL. In contrast, srebf1, tbx21, and gimap4 only showed limited discriminating abilities in different groups. There is a significant positive correlation between serum levels of S100A9 and NAFLD Activity Score (NAS), the severity of hepatic steatosis, and lobular inflammation (r = 0.80, 0.64 and 0.86, P < 0.001). These findings suggest that S100A9 may be extremely useful in the diagnosis of NASH (AUROC: 0.947, CI: 0.845-1.049). Additionally, serum S100A9 levels displayed a strong correlation with ALT, AST and TBil (r = 0.81, 0.89 and 0.91, P < 0.001) but a weak correlation with FBG, HOMA-IR, TG, and TC (r = -0.41, -0.40, 0.47 and 0.49, P < 0.05). Conclusions: The results we provide here suggest that S100A9 may be useful as a biomarker for the hepatic and metabolic progression of NAFLD and the non-invasive diagnosis of NASH. Public Library of Science 2015-05-19 /pmc/articles/PMC4437778/ /pubmed/25993652 http://dx.doi.org/10.1371/journal.pone.0127352 Text en © 2015 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Xiaolin Wang, Yongfeng Ming, Yanan Song, Yanyan Zhang, Jingyi Chen, Xiaoyu Zeng, Minde Mao, Yimin S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis |
title | S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis |
title_full | S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis |
title_fullStr | S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis |
title_full_unstemmed | S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis |
title_short | S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis |
title_sort | s100a9: a potential biomarker for the progression of non-alcoholic fatty liver disease and the diagnosis of non-alcoholic steatohepatitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437778/ https://www.ncbi.nlm.nih.gov/pubmed/25993652 http://dx.doi.org/10.1371/journal.pone.0127352 |
work_keys_str_mv | AT liuxiaolin s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT wangyongfeng s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT mingyanan s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT songyanyan s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT zhangjingyi s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT chenxiaoyu s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT zengminde s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis AT maoyimin s100a9apotentialbiomarkerfortheprogressionofnonalcoholicfattyliverdiseaseandthediagnosisofnonalcoholicsteatohepatitis |