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Experimental colitis in mice is attenuated by topical administration of chlorogenic acid

Epidemiological data suggest that the consumption of polyphenol-rich foods reduces the incidence of cancer, coronary heart disease, and inflammation. Chlorogenic acid (CGA), an ester of caffeic and quinic acids, is one of the most abundant polyphenol compounds in human diet with proven biological ef...

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Autores principales: Zatorski, Hubert, Sałaga, Maciej, Zielińska, Marta, Piechota-Polańczyk, Aleksandra, Owczarek, Katarzyna, Kordek, Radzisław, Lewandowska, Urszula, Chen, Chunqiu, Fichna, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438256/
https://www.ncbi.nlm.nih.gov/pubmed/25743575
http://dx.doi.org/10.1007/s00210-015-1110-9
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author Zatorski, Hubert
Sałaga, Maciej
Zielińska, Marta
Piechota-Polańczyk, Aleksandra
Owczarek, Katarzyna
Kordek, Radzisław
Lewandowska, Urszula
Chen, Chunqiu
Fichna, Jakub
author_facet Zatorski, Hubert
Sałaga, Maciej
Zielińska, Marta
Piechota-Polańczyk, Aleksandra
Owczarek, Katarzyna
Kordek, Radzisław
Lewandowska, Urszula
Chen, Chunqiu
Fichna, Jakub
author_sort Zatorski, Hubert
collection PubMed
description Epidemiological data suggest that the consumption of polyphenol-rich foods reduces the incidence of cancer, coronary heart disease, and inflammation. Chlorogenic acid (CGA), an ester of caffeic and quinic acids, is one of the most abundant polyphenol compounds in human diet with proven biological effectiveness both in vitro and in vivo. The aim of the study is to investigate the possible anti-inflammatory effect of CGA in the gastrointestinal (GI) tract and its mechanism of action. We used a well-established model of colitis, induced by intracolonic (i.c.) administration of trinitrobenzenesulfonic acid (TNBS) in mice. The anti-inflammatory effect of CGA in the colon was evaluated based on the clinical and macroscopic and microscopic parameters. To investigate the mechanism of protective action of CGA, myeloperoxidase (MPO), H(2)O(2), and NF-κB levels were assessed in the colon tissue. CGA administered i.c. at the dose of 20 mg/kg (two times daily) protected against TNBS-induced colitis more effectively than the same dose administered orally (p.o.), as evidenced by significantly lower macroscopic and ulcer scores. Furthermore, CGA (20 mg/kg, i.c.) reduced neutrophil infiltration, as demonstrated by decreased MPO activity. Moreover, CGA suppressed activation of NF-κB, as evidenced by lower levels of phospho-NF-κB/NF-κB ratio in the tissue. CGA did not affect the oxidative stress pathways. CGA exhibits anti-inflammatory properties through reduction of neutrophil infiltration and inhibition of NF-κB-dependent pathways. Our results suggest that CGA may have the potential to become a valuable supplement in the treatment of GI diseases.
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spelling pubmed-44382562015-05-20 Experimental colitis in mice is attenuated by topical administration of chlorogenic acid Zatorski, Hubert Sałaga, Maciej Zielińska, Marta Piechota-Polańczyk, Aleksandra Owczarek, Katarzyna Kordek, Radzisław Lewandowska, Urszula Chen, Chunqiu Fichna, Jakub Naunyn Schmiedebergs Arch Pharmacol Original Article Epidemiological data suggest that the consumption of polyphenol-rich foods reduces the incidence of cancer, coronary heart disease, and inflammation. Chlorogenic acid (CGA), an ester of caffeic and quinic acids, is one of the most abundant polyphenol compounds in human diet with proven biological effectiveness both in vitro and in vivo. The aim of the study is to investigate the possible anti-inflammatory effect of CGA in the gastrointestinal (GI) tract and its mechanism of action. We used a well-established model of colitis, induced by intracolonic (i.c.) administration of trinitrobenzenesulfonic acid (TNBS) in mice. The anti-inflammatory effect of CGA in the colon was evaluated based on the clinical and macroscopic and microscopic parameters. To investigate the mechanism of protective action of CGA, myeloperoxidase (MPO), H(2)O(2), and NF-κB levels were assessed in the colon tissue. CGA administered i.c. at the dose of 20 mg/kg (two times daily) protected against TNBS-induced colitis more effectively than the same dose administered orally (p.o.), as evidenced by significantly lower macroscopic and ulcer scores. Furthermore, CGA (20 mg/kg, i.c.) reduced neutrophil infiltration, as demonstrated by decreased MPO activity. Moreover, CGA suppressed activation of NF-κB, as evidenced by lower levels of phospho-NF-κB/NF-κB ratio in the tissue. CGA did not affect the oxidative stress pathways. CGA exhibits anti-inflammatory properties through reduction of neutrophil infiltration and inhibition of NF-κB-dependent pathways. Our results suggest that CGA may have the potential to become a valuable supplement in the treatment of GI diseases. Springer Berlin Heidelberg 2015-03-07 2015 /pmc/articles/PMC4438256/ /pubmed/25743575 http://dx.doi.org/10.1007/s00210-015-1110-9 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Zatorski, Hubert
Sałaga, Maciej
Zielińska, Marta
Piechota-Polańczyk, Aleksandra
Owczarek, Katarzyna
Kordek, Radzisław
Lewandowska, Urszula
Chen, Chunqiu
Fichna, Jakub
Experimental colitis in mice is attenuated by topical administration of chlorogenic acid
title Experimental colitis in mice is attenuated by topical administration of chlorogenic acid
title_full Experimental colitis in mice is attenuated by topical administration of chlorogenic acid
title_fullStr Experimental colitis in mice is attenuated by topical administration of chlorogenic acid
title_full_unstemmed Experimental colitis in mice is attenuated by topical administration of chlorogenic acid
title_short Experimental colitis in mice is attenuated by topical administration of chlorogenic acid
title_sort experimental colitis in mice is attenuated by topical administration of chlorogenic acid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438256/
https://www.ncbi.nlm.nih.gov/pubmed/25743575
http://dx.doi.org/10.1007/s00210-015-1110-9
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