Cargando…
Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages
Fungi that have the enzymes cyanase and carbonic anhydrase show a limited capacity to detoxify cyanate, a fungicide employed by both plants and humans. Here, we describe a novel two-gene cluster that comprises duplicated cyanase and carbonic anhydrase copies, which we name the CCA gene cluster, trac...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438557/ https://www.ncbi.nlm.nih.gov/pubmed/25663439 http://dx.doi.org/10.1093/gbe/evv025 |
_version_ | 1782372354992635904 |
---|---|
author | Elmore, M. Holly McGary, Kriston L. Wisecaver, Jennifer H. Slot, Jason C. Geiser, David M. Sink, Stacy O’Donnell, Kerry Rokas, Antonis |
author_facet | Elmore, M. Holly McGary, Kriston L. Wisecaver, Jennifer H. Slot, Jason C. Geiser, David M. Sink, Stacy O’Donnell, Kerry Rokas, Antonis |
author_sort | Elmore, M. Holly |
collection | PubMed |
description | Fungi that have the enzymes cyanase and carbonic anhydrase show a limited capacity to detoxify cyanate, a fungicide employed by both plants and humans. Here, we describe a novel two-gene cluster that comprises duplicated cyanase and carbonic anhydrase copies, which we name the CCA gene cluster, trace its evolution across Ascomycetes, and examine the evolutionary dynamics of its spread among lineages of the Fusarium oxysporum species complex (hereafter referred to as the FOSC), a cosmopolitan clade of purportedly clonal vascular wilt plant pathogens. Phylogenetic analysis of fungal cyanase and carbonic anhydrase genes reveals that the CCA gene cluster arose independently at least twice and is now present in three lineages, namely Cochliobolus lunatus, Oidiodendron maius, and the FOSC. Genome-wide surveys within the FOSC indicate that the CCA gene cluster varies in copy number across isolates, is always located on accessory chromosomes, and is absent in FOSC’s closest relatives. Phylogenetic reconstruction of the CCA gene cluster in 163 FOSC strains from a wide variety of hosts suggests a recent history of rampant transfers between isolates. We hypothesize that the independent formation of the CCA gene cluster in different fungal lineages and its spread across FOSC strains may be associated with resistance to plant-produced cyanates or to use of cyanate fungicides in agriculture. |
format | Online Article Text |
id | pubmed-4438557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44385572015-06-26 Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages Elmore, M. Holly McGary, Kriston L. Wisecaver, Jennifer H. Slot, Jason C. Geiser, David M. Sink, Stacy O’Donnell, Kerry Rokas, Antonis Genome Biol Evol Research Article Fungi that have the enzymes cyanase and carbonic anhydrase show a limited capacity to detoxify cyanate, a fungicide employed by both plants and humans. Here, we describe a novel two-gene cluster that comprises duplicated cyanase and carbonic anhydrase copies, which we name the CCA gene cluster, trace its evolution across Ascomycetes, and examine the evolutionary dynamics of its spread among lineages of the Fusarium oxysporum species complex (hereafter referred to as the FOSC), a cosmopolitan clade of purportedly clonal vascular wilt plant pathogens. Phylogenetic analysis of fungal cyanase and carbonic anhydrase genes reveals that the CCA gene cluster arose independently at least twice and is now present in three lineages, namely Cochliobolus lunatus, Oidiodendron maius, and the FOSC. Genome-wide surveys within the FOSC indicate that the CCA gene cluster varies in copy number across isolates, is always located on accessory chromosomes, and is absent in FOSC’s closest relatives. Phylogenetic reconstruction of the CCA gene cluster in 163 FOSC strains from a wide variety of hosts suggests a recent history of rampant transfers between isolates. We hypothesize that the independent formation of the CCA gene cluster in different fungal lineages and its spread across FOSC strains may be associated with resistance to plant-produced cyanates or to use of cyanate fungicides in agriculture. Oxford University Press 2015-02-06 /pmc/articles/PMC4438557/ /pubmed/25663439 http://dx.doi.org/10.1093/gbe/evv025 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Elmore, M. Holly McGary, Kriston L. Wisecaver, Jennifer H. Slot, Jason C. Geiser, David M. Sink, Stacy O’Donnell, Kerry Rokas, Antonis Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages |
title | Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages |
title_full | Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages |
title_fullStr | Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages |
title_full_unstemmed | Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages |
title_short | Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages |
title_sort | clustering of two genes putatively involved in cyanate detoxification evolved recently and independently in multiple fungal lineages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438557/ https://www.ncbi.nlm.nih.gov/pubmed/25663439 http://dx.doi.org/10.1093/gbe/evv025 |
work_keys_str_mv | AT elmoremholly clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT mcgarykristonl clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT wisecaverjenniferh clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT slotjasonc clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT geiserdavidm clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT sinkstacy clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT odonnellkerry clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages AT rokasantonis clusteringoftwogenesputativelyinvolvedincyanatedetoxificationevolvedrecentlyandindependentlyinmultiplefungallineages |