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In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa
The mammalian testis and epididymis are responsible for spermatozoa production and maturation, which contributes to male fertility. Predominantly expressed proteins in the testis and epididymis were suggested to be involved in the key functions or pathways in spermatogenesis and sperm maturation. To...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438928/ https://www.ncbi.nlm.nih.gov/pubmed/25760095 http://dx.doi.org/10.3892/mmr.2015.3435 |
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author | LIU, XUEXIA LIU, FUJUN |
author_facet | LIU, XUEXIA LIU, FUJUN |
author_sort | LIU, XUEXIA |
collection | PubMed |
description | The mammalian testis and epididymis are responsible for spermatozoa production and maturation, which contributes to male fertility. Predominantly expressed proteins in the testis and epididymis were suggested to be involved in the key functions or pathways in spermatogenesis and sperm maturation. To further investigate these proteins and their associations with sperm, large protein profiles of human testis and epididymis were mapped. Predominantly-expressed testicular (173) and epididymal (244) secreted proteins were further screened and functionally characterized. Differential expression levels of solute carrier family 2 (facilitated glucose transporter), member 3, solute carrier family 25 (carnitine/acylcarnitine translocase), member 20, WAP-type four-disulfide core domain protein 8 and prostate and testis expressed 1 were validated using western blot and immunohistochemical analyses. The results may provide novel insight into the understanding of testicular and epididymal physiology and function, and facilitate sperm maturation research. |
format | Online Article Text |
id | pubmed-4438928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-44389282015-06-05 In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa LIU, XUEXIA LIU, FUJUN Mol Med Rep Articles The mammalian testis and epididymis are responsible for spermatozoa production and maturation, which contributes to male fertility. Predominantly expressed proteins in the testis and epididymis were suggested to be involved in the key functions or pathways in spermatogenesis and sperm maturation. To further investigate these proteins and their associations with sperm, large protein profiles of human testis and epididymis were mapped. Predominantly-expressed testicular (173) and epididymal (244) secreted proteins were further screened and functionally characterized. Differential expression levels of solute carrier family 2 (facilitated glucose transporter), member 3, solute carrier family 25 (carnitine/acylcarnitine translocase), member 20, WAP-type four-disulfide core domain protein 8 and prostate and testis expressed 1 were validated using western blot and immunohistochemical analyses. The results may provide novel insight into the understanding of testicular and epididymal physiology and function, and facilitate sperm maturation research. D.A. Spandidos 2015-07 2015-03-05 /pmc/articles/PMC4438928/ /pubmed/25760095 http://dx.doi.org/10.3892/mmr.2015.3435 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles LIU, XUEXIA LIU, FUJUN In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
title | In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
title_full | In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
title_fullStr | In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
title_full_unstemmed | In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
title_short | In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
title_sort | in-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438928/ https://www.ncbi.nlm.nih.gov/pubmed/25760095 http://dx.doi.org/10.3892/mmr.2015.3435 |
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