Fluorescent-Protein Stabilization and High-Resolution Imaging of Cleared, Intact Mouse Brains

In order to observe and quantify long-range neuronal connections in intact mouse brain by light microscopy, it is first necessary to clear the brain, thus suppressing refractive-index variations. Here we describe a method that clears the brain and preserves the signal from proteinaceous fluorophores...

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Detalles Bibliográficos
Autores principales: Schwarz, Martin K., Scherbarth, Annemarie, Sprengel, Rolf, Engelhardt, Johann, Theer, Patrick, Giese, Guenter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439039/
https://www.ncbi.nlm.nih.gov/pubmed/25993380
http://dx.doi.org/10.1371/journal.pone.0124650

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439039/
https://www.ncbi.nlm.nih.gov/pubmed/25993380
http://dx.doi.org/10.1371/journal.pone.0124650

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