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Fluorescent-Protein Stabilization and High-Resolution Imaging of Cleared, Intact Mouse Brains
In order to observe and quantify long-range neuronal connections in intact mouse brain by light microscopy, it is first necessary to clear the brain, thus suppressing refractive-index variations. Here we describe a method that clears the brain and preserves the signal from proteinaceous fluorophores...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439039/ https://www.ncbi.nlm.nih.gov/pubmed/25993380 http://dx.doi.org/10.1371/journal.pone.0124650 |