Quantitative Localization Microscopy: Effects of Photophysics and Labeling Stoichiometry

Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average nu...

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Detalles Bibliográficos
Autores principales: Nieuwenhuizen, Robert P. J., Bates, Mark, Szymborska, Anna, Lidke, Keith A., Rieger, Bernd, Stallinga, Sjoerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439177/
https://www.ncbi.nlm.nih.gov/pubmed/25992915
http://dx.doi.org/10.1371/journal.pone.0127989

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439177/
https://www.ncbi.nlm.nih.gov/pubmed/25992915
http://dx.doi.org/10.1371/journal.pone.0127989

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